GSE102133

Mucosal gene expression profiling in ileum of patients with Crohn's disease

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

65 case / 12 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

In this study, we investigated gene expression profiles in ileal mucosa from CD patients in different settings (post-operative CD (Rutgeerts score i0); post-operative recurrent CD (i1), post-operative recurrent CD (≥i2b), newly diagnosed CD and late stage CD)) and controls.

Overall design

Ileal mucosal biopsies were obtained during endoscopy from 65 CD (3 i0 CD, 6 post-operative recurrent (POR) i1 CD, 24 POR (≥i2b), 18 new and 14 late) patients and 12 normal controls. Total RNA was isolated from ileal mucosal biopsies, labelled and hybridized to Affymetrix Genechip Human Gene 1.0ST Arrays.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
77 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Curated expression experiment linkage and source URL.

Expression Atlas accession
E-GEOD-102133
Available
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

9 linked publications 9 DOIs

Primary publication

  1. Gene and Mirna Regulatory Networks During Different Stages of Crohn's Disease. Journal of Crohn's & colitis, 2019

Related publications

  1. CrohnTwin-X: An explainable digital twin framework for predicting postoperative recurrence in Crohn's disease using multi-omics and clinical features. Computer methods and programs in biomedicine, 2026
  2. The critical role of COL1A1 revealed by integrated bioinformatics analysis of differentially-expressed genes in colorectal cancer and inflammatory bowel disease. Computers in biology and medicine, 2025
  3. Uncovering common disease mechanisms and critical biomarkers in Crohn's disease with concurrent psoriasis and exploring potential therapeutic agents. PloS one, 2025
  4. Identifying hub genes and miRNAs in Crohn's disease by bioinformatics analysis. Frontiers in genetics, 2022
  5. Identification of gene signatures associated with ulcerative colitis and the association with immune infiltrates in colon cancer. Frontiers in immunology, 2023
  6. Analysis of ferroptosis-associated genes in Crohn's disease based on bioinformatics. Frontiers in medicine, 2022
  7. Development and Experimental Validation of Machine Learning-Based Disulfidptosis-Related Ferroptosis Biomarkers in Inflammatory Bowel Disease. Genes, 2025
  8. Identification of differentially expressed genes associated with ferroptosis in Crohn's disease. Experimental and therapeutic medicine, 2024
Available

Metadata Quality Components

Analysis Readiness

Ready after review
97% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Crohn's disease Disease context came from Normalization rules.
  • Likely case group Crohn's disease 65 samples
  • Likely control group Healthy control 12 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded assay: microarray cohorts are separated from the RNA-seq intent. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Biopsy. Comparison suitability: 0%.
  • Interpreted disease metadata: Crohn's disease.
  • Processed data is available for downstream review.
  • External evidence: Expression Atlas curated experiment link is available.
  • External evidence: PubMed links 9 publications; 9 DOIs available: PMID 30657881, PMID 36118873, PMID 36714107, PMID 36742294, PMID 38274342.
  • Publication context: Gene and Mirna Regulatory Networks During Different Stages of Crohn's Disease.. Journal of Crohn's & colitis, 2019. PMID 30657881. DOI 10.1093/ecco-jcc/jjz007.
  • Publication context: Identifying hub genes and miRNAs in Crohn's disease by bioinformatics analysis.. Frontiers in genetics, 2022. PMID 36118873. DOI 10.3389/fgene.2022.950136.
  • Publication context: Analysis of ferroptosis-associated genes in Crohn's disease based on bioinformatics.. Frontiers in medicine, 2022. PMID 36714107. DOI 10.3389/fmed.2022.1058076.
  • Publication context: 6 additional linked PubMed publications are available.

Cautions

  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Microarray expression context downgraded below primary bulk RNA-seq for the default tissue ranking.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Overall score capped because microarray cohorts should be compared separately from RNA-seq cohorts.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

389 fields

Sample Group Preview

showing 62 of 77 preview rows
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Study totals: Case 65 | Control 12 | Unassigned 0 | Excluded 0 | Ambiguous 0 showing 62 of 77 preview rows
Group Role Accession Title Source / characteristics Reason
Crohn's disease Case GSM2728662 i0_CD_01 Biopsy from ileal mucosa of a post-operative CD patient (Rutgeert score i0) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728663 i0_CD_02 Biopsy from ileal mucosa of a post-operative CD patient (Rutgeert score i0) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728664 i0_CD_03 Biopsy from ileal mucosa of a post-operative CD patient (Rutgeert score i0) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728665 Late_CD_01 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728666 Late_CD_02 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728667 Late_CD_03 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728668 Late_CD_04 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728669 Late_CD_05 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728670 Late_CD_06 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728671 Late_CD_07 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728672 Late_CD_08 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728673 Late_CD_09 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728674 Late_CD_10 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728675 Late_CD_11 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728676 Late_CD_12 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728677 Late_CD_13 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728678 Late_CD_14 Biopsy from ileal mucosa of a late stage CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728679 New_CD_01 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728680 New_CD_02 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728681 New_CD_03 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728682 New_CD_04 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728683 New_CD_05 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728684 New_CD_06 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728685 New_CD_07 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728686 New_CD_08 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728687 New_CD_09 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728688 New_CD_10 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728689 New_CD_11 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728690 New_CD_12 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728691 New_CD_13 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728692 New_CD_14 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728693 New_CD_15 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728694 New_CD_16 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728695 New_CD_17 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728696 New_CD_18 Biopsy from ileal mucosa of a newly diagnosed CD patient Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728697 POR_CD_01 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728698 POR_CD_02 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728699 POR_CD_03 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728700 POR_CD_04 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728701 POR_CD_05 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728702 POR_CD_06 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728703 POR_CD_07 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728704 POR_CD_08 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728705 POR_CD_09 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728706 POR_CD_10 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728707 POR_CD_11 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728708 POR_CD_12 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728709 POR_CD_13 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728710 POR_CD_14 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
Crohn's disease Case GSM2728711 POR_CD_15 Biopsy from ileal mucosa of a post-operative recurrent CD patient (Rutgeerts score ≥i2b) Disease: Crohn's disease; Tissue: Ileum Case marker detected.
15 additional case samples are hidden from this preview.
Healthy control Control GSM2728650 Control_01 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728651 Control_02 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728652 Control_03 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728653 Control_04 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728654 Control_05 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728655 Control_06 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728656 Control_07 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728657 Control_08 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728658 Control_09 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728659 Control_10 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728660 Control_11 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.
Healthy control Control GSM2728661 Control_12 Biopsy from normal ileal mucosa of control individual Disease: Normal; Tissue: Ileum Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.