GSE10616

Human colon expression in healthy controls, colon-only CD, ileo-colonic CD, and UC

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

42 case / 11 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

Colon gene expression in human IBD. The three major clinical subsets of Inflammatory Bowel Disease (IBD) include colon-only Crohn's Disease (CD), ileo-colonic CD, and Ulcerative Colitis (UC). These experiments tested differential colon gene expression in these three types of IBD, relative to healthy control samples, and the local degree of mucosal inflammation as measured by the CD Histological Index of Severity (CDHIS). Colon biopsy samples were obtained from IBD patients at diagnosis and during therapy, and healthy controls. The global pattern of gene expression was determined using GeneSpring software, with a focus upon candidate genes identified in a recent genome wide association study in pediatric onset IBD. Data suggested that two of these candidate genes are up regulated in pediatric IBD, partially influenced by local mucosal inflammation. These experiments tested differential colon gene expression in healthy, CD, and UC samples for candidate genes identified in a recent pediatric onset IBD genome wide association study. Keywords: Single time point in CD and UC and healthy controls.

Overall design

Colon RNA was isolated from biopsies obtained from CD and UC at diagnosis and during therapy and healthy controls. Samples were obtained from the most proximal affected segment of colon. Microarray experiments were performed as described in the CCHMC microarray core, and data was analyzed as described above in the summary. The '107' internal control CEL files (for batches 1,2,3,4,5) used for normalization of the Sample VALUEs are also contained within this data set.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
58 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

7 linked publications 7 DOIs

Primary publication

  1. Loci on 20q13 and 21q22 are associated with pediatric-onset inflammatory bowel disease. Nature genetics, 2008

Related publications

  1. Identification of LPCAT1 as a key biomarker for Crohn's disease based on bioinformatics and machine learnings and experimental verification. Gene, 2024
  2. Molecular Comparison of Adult and Pediatric Ulcerative Colitis Indicates Broad Similarity of Molecular Pathways in Disease Tissue. Journal of pediatric gastroenterology and nutrition, 2018
  3. Integrated analysis of multiple microarray studies to establish differential diagnostic models of Crohn's disease and ulcerative colitis based on a metalloproteinase-associated module. Frontiers in immunology, 2022
  4. Identification of shared gene signatures and molecular mechanisms between chronic kidney disease and ulcerative colitis. Frontiers in immunology, 2023
  5. Machine learning-based solution reveals cuproptosis features in inflammatory bowel disease. Frontiers in immunology, 2023
  6. Advances in Inflammatory Bowel Disease Diagnostics: Machine Learning and Genomic Profiling Reveal Key Biomarkers for Early Detection. Diagnostics (Basel, Switzerland), 2024
Available

Metadata Quality Components

Analysis Readiness

Ready after review
94% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

12/15 pts

All non-excluded samples are assigned; excluded samples should still be confirmed.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

4/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Needs review

5 samples have hard exclusion flags in the proposed grouping.

Confirm excluded samples and preserve the reason in the analysis handoff.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Crohn's disease, inflammatory bowel disease, ulcerative colitis Disease context came from Normalization rules.
  • Likely case group Crohn's disease 42 samples
  • Likely control group Healthy control 11 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded assay: microarray cohorts are separated from the RNA-seq intent. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Tissue. Comparison suitability: 0%.
  • Interpreted disease metadata: Crohn's disease, Inflammatory bowel disease, Ulcerative colitis.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 7 publications; 7 DOIs available: PMID 18758464, PMID 29401083, PMID 36479126, PMID 36860851, PMID 37275904.
  • Publication context: Loci on 20q13 and 21q22 are associated with pediatric-onset inflammatory bowel disease.. Nature genetics, 2008. PMID 18758464. DOI 10.1038/ng.203.
  • Publication context: Molecular Comparison of Adult and Pediatric Ulcerative Colitis Indicates Broad Similarity of Molecular Pathways in Disease Tissue.. Journal of pediatric gastroenterology and nutrition, 2018. PMID 29401083. DOI 10.1097/MPG.0000000000001898.
  • Publication context: Integrated analysis of multiple microarray studies to establish differential diagnostic models of Crohn's disease and ulcerative colitis based on a metalloproteinase-associated module.. Frontiers in immunology, 2022. PMID 36479126. DOI 10.3389/fimmu.2022.1022850.
  • Publication context: 4 additional linked PubMed publications are available.

Cautions

  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Microarray expression context downgraded below primary bulk RNA-seq for the default tissue ranking.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Overall score capped because microarray cohorts should be compared separately from RNA-seq cohorts.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

197 fields

Sample Group Preview

58 rows shown
Show Hide
Study totals: Case 42 | Control 11 | Unassigned 0 | Excluded 5 | Ambiguous 0 58 rows shown
Group Role Accession Title Source / characteristics Reason
Crohn's disease Case GSM267527 Colon-only CD repl1 Human colon Colon-only crohn's disease replicate sample; Batch1 Case marker detected.
Crohn's disease Case GSM267528 Colon-only CD repl2 Human colon Colon-only crohn's disease replicate sample; Batch1 Case marker detected.
Crohn's disease Case GSM267529 Colon-only CD repl3 Human colon Colon-only crohn's disease replicate sample; Batch1 Case marker detected.
Crohn's disease Case GSM267530 Colon-only CD repl4 Human colon Colon-only crohn's disease replicate sample; Batch1 Case marker detected.
Crohn's disease Case GSM267531 Colon-only CD repl5 Human colon Colon-only crohn's disease replicate sample; Batch1 Case marker detected.
Crohn's disease Case GSM267532 Colon-only CD repl6 Human colon Colon-only crohn's disease replicate sample; Batch5 Case marker detected.
Crohn's disease Case GSM267533 Colon-only CD repl7 Human colon Colon-only crohn's disease replicate sample; Batch5 Case marker detected.
Crohn's disease Case GSM267534 Colon-only CD repl8 Human colon Colon-only crohn's disease replicate sample; Batch5 Case marker detected.
Crohn's disease Case GSM267535 Colon-only CD repl9 Human colon Colon-only crohn's disease replicate sample; Batch5 Case marker detected.
Crohn's disease Case GSM267536 Colon-only CD repl10 Human colon Colon-only crohn's disease replicate sample; Batch5 Case marker detected.
Crohn's disease Case GSM267537 Colon-only CD repl11 Human colon Colon-only crohn's disease replicate sample; Batch3 Case marker detected.
Crohn's disease Case GSM267538 Colon-only CD repl12 Human colon Colon-only crohn's disease replicate sample; Batch4 Case marker detected.
Crohn's disease Case GSM267539 Colon-only CD repl13 Human colon Colon-only crohn's disease replicate sample; Batch4 Case marker detected.
Crohn's disease Case GSM267540 Colon-only CD repl14 Human colon Colon-only crohn's disease replicate sample; Batch4 Case marker detected.
Crohn's disease Case GSM267541 Ileo-colonic CD repl1 Human colon Ileo-colonic crohn's disease replicate sample; Batch2 Case marker detected.
Crohn's disease Case GSM267542 Ileo-colonic CD repl2 Human colon Ileo-colonic crohn's disease replicate sample; Batch1 Case marker detected.
Crohn's disease Case GSM267543 Ileo-colonic CD repl3 Human colon Ileo-colonic crohn's disease replicate sample; Batch1 Case marker detected.
Crohn's disease Case GSM267544 Ileo-colonic CD repl4 Human colon Ileo-colonic crohn's disease replicate sample; Batch3 Case marker detected.
Crohn's disease Case GSM267545 Ileo-colonic CD repl5 Human colon Ileo-colonic crohn's disease replicate sample; Batch4 Case marker detected.
Crohn's disease Case GSM267546 Ileo-colonic CD repl6 Human colon Ileo-colonic crohn's disease replicate sample; Batch4 Case marker detected.
Crohn's disease Case GSM267547 Ileo-colonic CD repl7 Human colon Ileo-colonic crohn's disease replicate sample; Batch5 Case marker detected.
Crohn's disease Case GSM267548 Ileo-colonic CD repl8 Human colon Ileo-colonic crohn's disease replicate sample; Batch5 Case marker detected.
Crohn's disease Case GSM267549 Ileo-colonic CD repl9 Human colon Ileo-colonic crohn's disease replicate sample; Batch5 Case marker detected.
Crohn's disease Case GSM267550 Ileo-colonic CD repl10 Human colon Ileo-colonic crohn's disease replicate sample; Batch5 Case marker detected.
Crohn's disease Case GSM267551 Ileo-colonic CD repl11 Human colon Ileo-colonic crohn's disease replicate sample; Batch5 Case marker detected.
Crohn's disease Case GSM267552 Ileo-colonic CD repl12 Human colon Ileo-colonic crohn's disease replicate sample; Batch3 Case marker detected.
Crohn's disease Case GSM267553 Ileo-colonic CD repl13 Human colon Ileo-colonic crohn's disease replicate sample; Batch3 Case marker detected.
Crohn's disease Case GSM267554 Ileo-colonic CD repl14 Human colon Ileo-colonic crohn's disease replicate sample; Batch3 Case marker detected.
Crohn's disease Case GSM267555 Ileo-colonic CD repl15 Human colon Ileo-colonic crohn's disease replicate sample; Batch3 Case marker detected.
Crohn's disease Case GSM267556 Ileo-colonic CD repl16 Human colon Ileo-colonic crohn's disease replicate sample; Batch3 Case marker detected.
Crohn's disease Case GSM267557 Ileo-colonic CD repl17 Human colon Ileo-colonic crohn's disease replicate sample; Batch4 Case marker detected.
Crohn's disease Case GSM267558 Ileo-colonic CD repl18 Human colon Ileo-colonic crohn's disease replicate sample; Batch5 Case marker detected.
Ulcerative colitis Case GSM267575 Ulcerative colitis repl1 Human colon Ulcerative colitis replicate sample; Batch2 Case marker detected.
Ulcerative colitis Case GSM267576 Ulcerative colitis repl2 Human colon Ulcerative colitis replicate sample; Batch4 Case marker detected.
Ulcerative colitis Case GSM267577 Ulcerative colitis repl3 Human colon Ulcerative colitis replicate sample; Batch4 Case marker detected.
Ulcerative colitis Case GSM267578 Ulcerative colitis repl4 Human colon Ulcerative colitis replicate sample; Batch4 Case marker detected.
Ulcerative colitis Case GSM267579 Ulcerative colitis repl5 Human colon Ulcerative colitis replicate sample; Batch4 Case marker detected.
Ulcerative colitis Case GSM267580 Ulcerative colitis repl6 Human colon Ulcerative colitis replicate sample; Batch4 Case marker detected.
Ulcerative colitis Case GSM267581 Ulcerative colitis repl7 Human colon Ulcerative colitis replicate sample; Batch5 Case marker detected.
Ulcerative colitis Case GSM267582 Ulcerative colitis repl8 Human colon Ulcerative colitis replicate sample; Batch5 Case marker detected.
Ulcerative colitis Case GSM267583 Ulcerative colitis repl9 Human colon Ulcerative colitis replicate sample; Batch5 Case marker detected.
Ulcerative colitis Case GSM267584 Ulcerative colitis repl10 Human colon Ulcerative colitis replicate sample; Batch5 Case marker detected.
Healthy control Control GSM267564 Healthy control repl1 Human colon Healthy control replicate sample; Batch2 Sample-local control marker detected.
Healthy control Control GSM267565 Healthy control repl2 Human colon Healthy control replicate sample; Batch2 Sample-local control marker detected.
Healthy control Control GSM267566 Healthy control repl3 Human colon Healthy control replicate sample; Batch2 Sample-local control marker detected.
Healthy control Control GSM267567 Healthy control repl4 Human colon Healthy control replicate sample; Batch1 Sample-local control marker detected.
Healthy control Control GSM267568 Healthy control repl5 Human colon Healthy control replicate sample; Batch1 Sample-local control marker detected.
Healthy control Control GSM267569 Healthy control repl6 Human colon Healthy control replicate sample; Batch1 Sample-local control marker detected.
Healthy control Control GSM267570 Healthy control repl7 Human colon Healthy control replicate sample; Batch1 Sample-local control marker detected.
Healthy control Control GSM267571 Healthy control repl8 Human colon Healthy control replicate sample; Batch1 Sample-local control marker detected.
Healthy control Control GSM267572 Healthy control repl9 Human colon Healthy control replicate sample; Batch5 Sample-local control marker detected.
Healthy control Control GSM267573 Healthy control repl10 Human colon Healthy control replicate sample; Batch5 Sample-local control marker detected.
Healthy control Control GSM267574 Healthy control repl11 Human colon Healthy control replicate sample; Batch5 Sample-local control marker detected.
Excluded: Internal reference Excluded GSM267559 Internal control repl1 Human colon Internal control replicate sample; Batch5 internal reference: Internal or common reference sample metadata detected.
Excluded: Internal reference Excluded GSM267560 Internal control repl2 Human colon Internal control replicate sample; Batch2 internal reference: Internal or common reference sample metadata detected.
Excluded: Internal reference Excluded GSM267561 Internal control repl3 Human colon Internal control replicate sample; Batch1 internal reference: Internal or common reference sample metadata detected.
Excluded: Internal reference Excluded GSM267562 Internal control repl4 Human colon Internal control replicate sample; Batch3 internal reference: Internal or common reference sample metadata detected.
Excluded: Internal reference Excluded GSM267563 Internal control repl5 Human colon Internal control replicate sample; Batch4 internal reference: Internal or common reference sample metadata detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.