GSE142255

Gene expression profiling of immune cells from patients with acute on chronic liver failure (ACLF)

Whole-study status Exploratory only

Dataset can inform exploration but is not strong enough for primary analysis.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

Whole blood from 7 patients with stable cirrhosis, 7 patients with acutely decompensated cirrhosis without ACLF and 17 patients with ACLF (8 related to sepsis and 9 unrelated to sepsis) was sampled into tempus tubes (Applied biosystems, Ambion). The same method was used to sample whole blood from 7 healthy subjects and from 8 patients with septic shock without cirrhosis that were used as comparators.RNA was then extracted using the PerfectPure RNA blood kit (3 PRIME) according to manufactuter instructions. 100 ng of RNA per sample were then hybridized on Human Transcriptome Array 2.0

Overall design

Prospective trial comparing whole blood transcriptome of different group of patients according to the existance of chronic liver disease and to its severity

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
46 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

4 linked publications 4 DOIs

Primary publication

  1. Characterization of Blood Immune Cells in Patients With Decompensated Cirrhosis Including ACLF. Frontiers in immunology, 2020

Related publications

  1. Bioinformatics analyses of potential ACLF biological mechanisms and identification of immune-related hub genes and vital miRNAs. Scientific reports, 2022
  2. CD3D and CD247 are the molecular targets of septic shock. Medicine, 2023
  3. Identification and validation of neutrophil-related biomarkers in acute-on-chronic liver failure. Frontiers in immunology, 2025
Available

Metadata Quality Components

Analysis Readiness

Ready after review
97% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Exploratory only Dataset can inform exploration but is not strong enough for primary analysis.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Sepsis, septic shock Disease context came from Normalization rules.
  • Likely case group Patient with stable cirrhosis 39 samples
  • Likely control group Healthy control 7 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded for tissue search because it is a biofluid cohort. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Patient biofluid vs healthy control. Specimen: Biofluid. Comparison suitability: 20%.
  • Interpreted disease metadata: Sepsis, Septic shock.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 4 publications; 4 DOIs available: PMID 33613548, PMID 35982134, PMID 37478215, PMID 40070835.
  • Publication context: Characterization of Blood Immune Cells in Patients With Decompensated Cirrhosis Including ACLF.. Frontiers in immunology, 2020. PMID 33613548. DOI 10.3389/fimmu.2020.619039.
  • Publication context: Bioinformatics analyses of potential ACLF biological mechanisms and identification of immune-related hub genes and vital miRNAs.. Scientific reports, 2022. PMID 35982134. DOI 10.1038/s41598-022-18396-1.
  • Publication context: CD3D and CD247 are the molecular targets of septic shock.. Medicine, 2023. PMID 37478215. DOI 10.1097/MD.0000000000034295.
  • Publication context: 1 additional linked PubMed publications are available.

Cautions

  • Downgraded for tissue search because it is a biofluid cohort.
  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Blood, plasma, or serum cohort downgraded because this ranking targets tissue tumor-vs-normal comparisons.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

144 fields

Sample Group Preview

46 rows shown
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Study totals: Case 39 | Control 7 | Unassigned 0 | Excluded 0 | Ambiguous 0 46 rows shown
Group Role Accession Title Source / characteristics Reason
Patient with stable cirrhosis Case GSM4223781 P26 Stable decompensated cirrhosis Cell type: Whole blood; Stage of cirrhosis: Stable decompensated cirrhosis; Sepsis: No; Disease state: Patient with stable cirrhosis Explicit characteristic comparison arm detected.
Patient with stable cirrhosis Case GSM4223782 P27 Stable decompensated cirrhosis Cell type: Whole blood; Stage of cirrhosis: Stable decompensated cirrhosis; Sepsis: No; Disease state: Patient with stable cirrhosis Explicit characteristic comparison arm detected.
Patient with stable cirrhosis Case GSM4223783 P28 Stable decompensated cirrhosis Cell type: Whole blood; Stage of cirrhosis: Stable decompensated cirrhosis; Sepsis: No; Disease state: Patient with stable cirrhosis Explicit characteristic comparison arm detected.
Patient with stable cirrhosis Case GSM4223784 P31 Stable decompensated cirrhosis Cell type: Whole blood; Stage of cirrhosis: Stable decompensated cirrhosis; Sepsis: No; Disease state: Patient with stable cirrhosis Explicit characteristic comparison arm detected.
Patient with stable cirrhosis Case GSM4223785 P101 Stable decompensated cirrhosis Cell type: Whole blood; Stage of cirrhosis: Stable decompensated cirrhosis; Sepsis: No; Disease state: Patient with stable cirrhosis Explicit characteristic comparison arm detected.
Patient with stable cirrhosis Case GSM4223786 P111 Stable decompensated cirrhosis Cell type: Whole blood; Stage of cirrhosis: Stable decompensated cirrhosis; Sepsis: No; Disease state: Patient with stable cirrhosis Explicit characteristic comparison arm detected.
Patient with stable cirrhosis Case GSM4223787 P118 Stable decompensated cirrhosis Cell type: Whole blood; Stage of cirrhosis: Stable decompensated cirrhosis; Sepsis: No; Disease state: Patient with stable cirrhosis Explicit characteristic comparison arm detected.
Patient with stable cirrhosis Case GSM4223788 P25 Acute decompensation Cell type: Whole blood; Stage of cirrhosis: Acute decompensation; Sepsis: No; Disease state: Patient with stable cirrhosis Explicit characteristic comparison arm detected.
Patient with acutely decompensated cirrhosis Case GSM4223789 P29 Acute decompensation Cell type: Whole blood; Stage of cirrhosis: Acute decompensation; Sepsis: No; Disease state: Patient with acutely decompensated cirrhosis Explicit characteristic comparison arm detected.
Patient with acutely decompensated cirrhosis Case GSM4223790 P103 Acute decompensation Cell type: Whole blood; Stage of cirrhosis: Acute decompensation; Sepsis: No; Disease state: Patient with acutely decompensated cirrhosis Explicit characteristic comparison arm detected.
Patient with acutely decompensated cirrhosis Case GSM4223791 P105 Acute decompensation Cell type: Whole blood; Stage of cirrhosis: Acute decompensation; Sepsis: No; Disease state: Patient with acutely decompensated cirrhosis Explicit characteristic comparison arm detected.
Patient with acutely decompensated cirrhosis Case GSM4223792 P107 Acute decompensation Cell type: Whole blood; Stage of cirrhosis: Acute decompensation; Sepsis: No; Disease state: Patient with acutely decompensated cirrhosis Explicit characteristic comparison arm detected.
Patient with acutely decompensated cirrhosis Case GSM4223793 P116 Acute decompensation Cell type: Whole blood; Stage of cirrhosis: Acute decompensation; Sepsis: No; Disease state: Patient with acutely decompensated cirrhosis Explicit characteristic comparison arm detected.
Patient with acutely decompensated cirrhosis Case GSM4223794 P117 Acute decompensation Cell type: Whole blood; Stage of cirrhosis: Acute decompensation; Sepsis: No; Disease state: Patient with acutely decompensated cirrhosis Explicit characteristic comparison arm detected.
Sepsis or systemic inflammatory response Case GSM4223795 P1 Sepsis-induced ACLF Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: Yes; Disease state: Patient with ACLF that is related to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223796 P2 Sepsis-induced ACLF Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: Yes; Disease state: Patient with ACLF that is related to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223797 P3 Sepsis-induced ACLF Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: Yes; Disease state: Patient with ACLF that is related to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223798 P4 Sepsis-induced ACLF Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: Yes; Disease state: Patient with ACLF that is related to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223799 P6 Sepsis-induced ACLF Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: Yes; Disease state: Patient with ACLF that is related to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223800 P12 Sepsis-induced ACLF Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: Yes; Disease state: Patient with ACLF that is related to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223801 P19 Sepsis-induced ACLF Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: Yes; Disease state: Patient with ACLF that is related to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223802 P24 Sepsis-induced ACLF Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: Yes; Disease state: Patient with ACLF that is related to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223803 P9 ACLF unrelated to sepsis Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: No; Disease state: Patient with ACLF that is unrelated to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223804 P14 ACLF unrelated to sepsis Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: No; Disease state: Patient with ACLF that is unrelated to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223805 P15 ACLF unrelated to sepsis Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: No; Disease state: Patient with ACLF that is unrelated to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223806 P17 ACLF unrelated to sepsis Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: No; Disease state: Patient with ACLF that is unrelated to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223807 P30 ACLF unrelated to sepsis Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: No; Disease state: Patient with ACLF that is unrelated to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223808 P32 ACLF unrelated to sepsis Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: No; Disease state: Patient with ACLF that is unrelated to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223809 P104 ACLF unrelated to sepsis Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: No; Disease state: Patient with ACLF that is unrelated to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223810 P109 ACLF unrelated to sepsis Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: No; Disease state: Patient with ACLF that is unrelated to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223811 P112 ACLF unrelated to sepsis Cell type: Whole blood; Stage of cirrhosis: ACLF; Sepsis: No; Disease state: Patient with ACLF that is unrelated to sepsis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223812 P5 Septic shock W/O cirrhosis Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: Yes; Disease state: Patient with a septic shock but without cirrhosis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223813 P7 Septic shock W/O cirrhosis Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: Yes; Disease state: Patient with a septic shock but without cirrhosis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223814 P8 Septic shock W/O cirrhosis Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: Yes; Disease state: Patient with a septic shock but without cirrhosis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223815 P10 Septic shock W/O cirrhosis Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: Yes; Disease state: Patient with a septic shock but without cirrhosis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223816 P11 Septic shock W/O cirrhosis Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: Yes; Disease state: Patient with a septic shock but without cirrhosis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223817 P16 Septic shock W/O cirrhosis Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: Yes; Disease state: Patient with a septic shock but without cirrhosis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223818 P18 Septic shock W/O cirrhosis Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: Yes; Disease state: Patient with a septic shock but without cirrhosis Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM4223819 P21 Septic shock W/O cirrhosis Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: Yes; Disease state: Patient with a septic shock but without cirrhosis Explicit sample label case marker detected.
Healthy control Control GSM4223774 T2 Healthy subject Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: No; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM4223775 T3 Healthy subject Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: No; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM4223776 T4 Healthy subject Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: No; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM4223777 T5 Healthy subject Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: No; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM4223778 T6 Healthy subject Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: No; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM4223779 T7 Healthy subject Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: No; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM4223780 T8 Healthy subject Cell type: Whole blood; Stage of cirrhosis: No; Sepsis: No; Disease state: Healthy control Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.