GSE22889

Nitric oxide pathway specific gene alterations in inflammatory bowel disease

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

40 case / 4 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

Studying genes involved in the regulation of inflammation may help to clarify the pathogenesis of IBD. In search of the genes which products are known to be involved in NO signalling and altered in patients with IBD, our present aim was to determine the expression of such genes in CD and UC, respectively. To this end, microarray expression of genes known to be controlled by or which products are involved in NO signalling was studied in biopsies of colonic mucosa from patients and healthy controls.

Overall design

A cohort of inflamed colonic mucosa samples from 20 CD patients and 20 UC patients, as well as 6 control colonic mucosa samples, was used to acquire expression profiles. All of the inflamed samples were analysed individually, while the control samples were pooled and analysed in 4 replicates.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
44 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Publication context when PubMed identifiers are already known.

No match found

Metadata Quality Components

Analysis Readiness

Ready after review
97% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Inflammatory bowel disease Disease context came from Normalization rules.
  • Likely case group Inflammatory bowel disease 40 samples
  • Likely control group Control 4 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded assay: microarray cohorts are separated from the RNA-seq intent. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Tissue. Comparison suitability: 0%.
  • Interpreted disease metadata: Inflammatory bowel disease.
  • Processed data is available for downstream review.

Cautions

  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Microarray expression context downgraded below primary bulk RNA-seq for the default tissue ranking.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Overall score capped because microarray cohorts should be compared separately from RNA-seq cohorts.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

180 fields

Sample Group Preview

44 rows shown
Show Hide
Study totals: Case 40 | Control 4 | Unassigned 0 | Excluded 0 | Ambiguous 0 44 rows shown
Group Role Accession Title Source / characteristics Reason
Inflammatory bowel disease Case GSM565409 Colon_CD_2 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 30; Duration of disease: 5 months Case marker detected.
Inflammatory bowel disease Case GSM565410 Colon_CD_3 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Female; Age: 76; Duration of disease: Diagnosed at inclusion Case marker detected.
Inflammatory bowel disease Case GSM565411 Colon_CD_4 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 36; Duration of disease: 16 years Case marker detected.
Inflammatory bowel disease Case GSM565412 Colon_CD_6 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 53; Duration of disease: 5 years Case marker detected.
Inflammatory bowel disease Case GSM565413 Colon_CD_9 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 57; Duration of disease: 38 years Case marker detected.
Inflammatory bowel disease Case GSM565414 Colon_CD_10 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 23 Case marker detected.
Inflammatory bowel disease Case GSM565415 Colon_CD_11 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 53; Duration of disease: 21 years Case marker detected.
Inflammatory bowel disease Case GSM565416 Colon_CD_15 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Female; Age: 32; Duration of disease: 21 years Case marker detected.
Inflammatory bowel disease Case GSM565417 Colon_CD_16 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 43; Duration of disease: 3 years Case marker detected.
Inflammatory bowel disease Case GSM565418 Colon_CD_17 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 41; Duration of disease: 4 years Case marker detected.
Inflammatory bowel disease Case GSM565419 Colon_CD_18 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Female; Age: 38; Duration of disease: 16 years Case marker detected.
Inflammatory bowel disease Case GSM565420 Colon_CD_19 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Female; Age: 52; Duration of disease: 8 years Case marker detected.
Inflammatory bowel disease Case GSM565421 Colon_CD_1 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 24; Duration of disease: 9 years Case marker detected.
Inflammatory bowel disease Case GSM565422 Colon_CD_12 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 24; Duration of disease: 14 years Case marker detected.
Inflammatory bowel disease Case GSM565423 Colon_CD_14 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 58; Duration of disease: 30 years Case marker detected.
Inflammatory bowel disease Case GSM565424 Colon_CD_20 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Female; Age: 52; Duration of disease: 3 years Case marker detected.
Inflammatory bowel disease Case GSM565425 Colon_CD_21 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Female; Age: 47; Duration of disease: 23 years Case marker detected.
Inflammatory bowel disease Case GSM565426 Colon_CD_22 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Female; Age: 35; Duration of disease: 19 years Case marker detected.
Inflammatory bowel disease Case GSM565427 Colon_CD_23 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 70; Duration of disease: 31 years Case marker detected.
Inflammatory bowel disease Case GSM565428 Colon_CD_26 Colon mucosa, CD Tissue: Colon mucosa; Ibd subtype: CD; Sex: Male; Age: 67; Duration of disease: Diagnosed at inclusion Case marker detected.
Inflammatory bowel disease Case GSM565429 Colon_UC_2 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 32; Duration of disease: 2 years Case marker detected.
Inflammatory bowel disease Case GSM565430 Colon_UC_3 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Female; Age: 30; Duration of disease: 14 years Case marker detected.
Inflammatory bowel disease Case GSM565431 Colon_UC_4 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 31; Duration of disease: Diagnosed at inclusion Case marker detected.
Inflammatory bowel disease Case GSM565432 Colon_UC_5 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Female; Age: 27; Duration of disease: 15 years Case marker detected.
Inflammatory bowel disease Case GSM565433 Colon_UC_6 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 83; Duration of disease: Diagnosed at inclusion Case marker detected.
Inflammatory bowel disease Case GSM565434 Colon_UC_7 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Female; Age: 38; Duration of disease: 32 years Case marker detected.
Inflammatory bowel disease Case GSM565435 Colon_UC_11 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 26; Duration of disease: Diagnosed at inclusion Case marker detected.
Inflammatory bowel disease Case GSM565436 Colon_UC_12 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 39; Duration of disease: 28 years Case marker detected.
Inflammatory bowel disease Case GSM565437 Colon_UC_13 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Female; Age: 49; Duration of disease: 6 years Case marker detected.
Inflammatory bowel disease Case GSM565438 Colon_UC_14 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 35; Duration of disease: Diagnosed at inclusion Case marker detected.
Inflammatory bowel disease Case GSM565439 Colon_UC_1 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 57; Duration of disease: 36 years Case marker detected.
Inflammatory bowel disease Case GSM565440 Colon_UC_10 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 64; Duration of disease: 45 years Case marker detected.
Inflammatory bowel disease Case GSM565441 Colon_UC_15 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 50; Duration of disease: 12 years Case marker detected.
Inflammatory bowel disease Case GSM565442 Colon_UC_16 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Female; Age: 46; Duration of disease: 16 years Case marker detected.
Inflammatory bowel disease Case GSM565443 Colon_UC_17 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Female; Age: 31; Duration of disease: 6 years Case marker detected.
Inflammatory bowel disease Case GSM565444 Colon_UC_18 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 24; Duration of disease: 2 years Case marker detected.
Inflammatory bowel disease Case GSM565445 Colon_UC_19 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Female; Age: 23; Duration of disease: Diagnosed at inclusion Case marker detected.
Inflammatory bowel disease Case GSM565446 Colon_UC_20 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Female; Age: 33; Duration of disease: 21 years Case marker detected.
Inflammatory bowel disease Case GSM565447 Colon_UC_8 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Male; Age: 17; Duration of disease: 5 months Case marker detected.
Inflammatory bowel disease Case GSM565448 Colon_UC_9 Colon mucosa, UC Tissue: Colon mucosa; Ibd subtype: UC; Sex: Female; Age: 50; Duration of disease: Diagnosed at inclusion Case marker detected.
Control Control GSM565405 Colon_control_rep1 Colon mucosa, control Tissue: Colon mucosa; Ibd subtype: Control; Sex: 2 males, 4 females; Age: 20-77 Sample-local control marker detected.
Control Control GSM565406 Colon_control_rep2 Colon mucosa, control Tissue: Colon mucosa; Ibd subtype: Control; Sex: 2 males, 4 females; Age: 20-77 Sample-local control marker detected.
Control Control GSM565407 Colon_control_rep3 Colon mucosa, control Tissue: Colon mucosa; Ibd subtype: Control; Sex: 2 males, 4 females; Age: 20-77 Sample-local control marker detected.
Control Control GSM565408 Colon_control_rep4 Colon mucosa, control Tissue: Colon mucosa; Ibd subtype: Control; Sex: 2 males, 4 females; Age: 20-77 Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.