GSE294225

Transcriptional profiles are dramatically altered in circulating CD14+ monocytes in Rheumatoid Arthritis [RNA-seq]

Whole-study status Exploratory only

Dataset can inform exploration but is not strong enough for primary analysis.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

9 case / 15 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

Peripheral blood samples were collected from healthy controls and rheumatoid arthritis patients (active disease: DAS28 > 2.7), subsequently CD14+ monocytes were isolated using a positive selection kit, RNA was extracted and RNA-Seq was completed.

Overall design

RNA-Seq using the illumina NovaSeq6000 was performed on circulating CD14+ monocytes isolated from 15 healthy controls and 9 rheumatoid arthritis patients.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
24 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

1 linked publication 1 DOI

Primary publication

  1. Investigating endogenous immune-mediated monocyte memory in rheumatoid arthritis. Annals of the rheumatic diseases, 2025
Available

Metadata Quality Components

Analysis Readiness

Ready after review
100% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

10/10 pts

No dataset warnings are present.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Standard analyst review

Proceed only after the analyst confirms grouping, exclusions, and source files.

By Siftome standards, no expert review was triggered.

Why This Is Ranked Here

Tumor vs normal
Exploratory only Dataset can inform exploration but is not strong enough for primary analysis.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Disease context Rheumatoid arthritis Disease context came from Normalization rules.
  • Likely case group Rheumatoid arthritis 9 samples
  • Likely control group Healthy control 15 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded for tissue search because it is a biofluid cohort. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Patient biofluid vs healthy control. Specimen: Biofluid. Comparison suitability: 20%.
  • Prioritized assay: bulk RNA-seq is the active assay expectation.
  • Interpreted disease metadata: Rheumatoid arthritis.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 1 publication; 1 DOI available: PMID 40350372.
  • Publication context: Investigating endogenous immune-mediated monocyte memory in rheumatoid arthritis.. Annals of the rheumatic diseases, 2025. PMID 40350372. DOI 10.1016/j.ard.2025.03.016.

Cautions

  • Downgraded for tissue search because it is a biofluid cohort.
  • Blood, plasma, or serum cohort downgraded because this ranking targets tissue tumor-vs-normal comparisons.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

No dataset warnings

Normalized Fields

130 fields

Sample Group Preview

24 rows shown
Show Hide
Study totals: Case 9 | Control 15 | Unassigned 0 | Excluded 0 | Ambiguous 0 24 rows shown
Group Role Accession Title Source / characteristics Reason
Rheumatoid arthritis Case GSM8900587 Peripheral blood CD14+ monocyte, Rheumatoid Arthritis, Rep1 Peripheral Blood Tissue: Peripheral Blood; Subject status: Rheumatoid Arthritis; Cell type: Monocyte Case marker detected.
Rheumatoid arthritis Case GSM8900588 Peripheral blood CD14+ monocyte, Rheumatoid Arthritis, Rep2 Peripheral Blood Tissue: Peripheral Blood; Subject status: Rheumatoid Arthritis; Cell type: Monocyte Case marker detected.
Rheumatoid arthritis Case GSM8900589 Peripheral blood CD14+ monocyte, Rheumatoid Arthritis, Rep3 Peripheral Blood Tissue: Peripheral Blood; Subject status: Rheumatoid Arthritis; Cell type: Monocyte Case marker detected.
Rheumatoid arthritis Case GSM8900590 Peripheral blood CD14+ monocyte, Rheumatoid Arthritis, Rep4 Peripheral Blood Tissue: Peripheral Blood; Subject status: Rheumatoid Arthritis; Cell type: Monocyte Case marker detected.
Rheumatoid arthritis Case GSM8900591 Peripheral blood CD14+ monocyte, Rheumatoid Arthritis, Rep5 Peripheral Blood Tissue: Peripheral Blood; Subject status: Rheumatoid Arthritis; Cell type: Monocyte Case marker detected.
Rheumatoid arthritis Case GSM8900592 Peripheral blood CD14+ monocyte, Rheumatoid Arthritis, Rep6 Peripheral Blood Tissue: Peripheral Blood; Subject status: Rheumatoid Arthritis; Cell type: Monocyte Case marker detected.
Rheumatoid arthritis Case GSM8900593 Peripheral blood CD14+ monocyte, Rheumatoid Arthritis, Rep7 Peripheral Blood Tissue: Peripheral Blood; Subject status: Rheumatoid Arthritis; Cell type: Monocyte Case marker detected.
Rheumatoid arthritis Case GSM8900594 Peripheral blood CD14+ monocyte, Rheumatoid Arthritis, Rep8 Peripheral Blood Tissue: Peripheral Blood; Subject status: Rheumatoid Arthritis; Cell type: Monocyte Case marker detected.
Rheumatoid arthritis Case GSM8900595 Peripheral blood CD14+ monocyte, Rheumatoid Arthritis, Rep9 Peripheral Blood Tissue: Peripheral Blood; Subject status: Rheumatoid Arthritis; Cell type: Monocyte Case marker detected.
Healthy control Control GSM8900572 Peripheral blood CD14+ monocyte, Healthy Control, Rep1 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900573 Peripheral blood CD14+ monocyte, Healthy Control, Rep2 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900574 Peripheral blood CD14+ monocyte, Healthy Control, Rep3 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900575 Peripheral blood CD14+ monocyte, Healthy Control, Rep4 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900576 Peripheral blood CD14+ monocyte, Healthy Control, Rep5 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900577 Peripheral blood CD14+ monocyte, Healthy Control, Rep6 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900578 Peripheral blood CD14+ monocyte, Healthy Control, Rep7 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900579 Peripheral blood CD14+ monocyte, Healthy Control, Rep8 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900580 Peripheral blood CD14+ monocyte, Healthy Control, Rep9 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900581 Peripheral blood CD14+ monocyte, Healthy Control, Rep10 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900582 Peripheral blood CD14+ monocyte, Healthy Control, Rep11 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900583 Peripheral blood CD14+ monocyte, Healthy Control, Rep12 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900584 Peripheral blood CD14+ monocyte, Healthy Control, Rep13 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900585 Peripheral blood CD14+ monocyte, Healthy Control, Rep14 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.
Healthy control Control GSM8900586 Peripheral blood CD14+ monocyte, Healthy Control, Rep15 Peripheral Blood Tissue: Peripheral Blood; Subject status: Healthy Control; Cell type: Monocyte Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.