GSE294985

Transcriptomes of human whole blood cells in sepsis patients

Whole-study status Exploratory only

Dataset can inform exploration but is not strong enough for primary analysis.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

This study focuses on the transcriptomic characteristics of whole blood cells in sepsis patients. Sepsis is a severe systemic infection that often leads to multiple organ dysfunction. Its pathogenesis is complex and involves the abnormal activation of various cellular and molecular pathways. By analyzing the transcriptomes of whole blood cells in sepsis patients through sequencing, we aim to reveal the key changes in gene expression and their potential regulatory mechanisms during the onset and progression of sepsis. The study included sepsis patients with different underlying diseases and also set up a healthy control group for comparative analysis.

Overall design

RNA-seq profiling from the whole blood cells from 10 healthy controls and 30 sepsis patients.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
40 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

1 linked publication 1 DOI

Primary publication

  1. Construction of a robust sepsis prognostic classifier based on E3 ubiquitin ligase-related genes. Frontiers in molecular biosciences, 2026
Available

Metadata Quality Components

Analysis Readiness

Ready after review
100% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

10/10 pts

No dataset warnings are present.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Standard analyst review

Proceed only after the analyst confirms grouping, exclusions, and source files.

By Siftome standards, no expert review was triggered.

Why This Is Ranked Here

Tumor vs normal
Exploratory only Dataset can inform exploration but is not strong enough for primary analysis.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Disease context Sepsis Disease context came from Normalization rules.
  • Likely case group Sepsis or systemic inflammatory response 30 samples
  • Likely control group Healthy control 10 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded for tissue search because it is a biofluid cohort. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Patient biofluid vs healthy control. Specimen: Biofluid. Comparison suitability: 20%.
  • Prioritized assay: bulk RNA-seq is the active assay expectation.
  • Interpreted disease metadata: Infectious disease or host response, Sepsis.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 1 publication; 1 DOI available: PMID 41783141.
  • Publication context: Construction of a robust sepsis prognostic classifier based on E3 ubiquitin ligase-related genes.. Frontiers in molecular biosciences, 2026. PMID 41783141. DOI 10.3389/fmolb.2026.1726356.

Cautions

  • Downgraded for tissue search because it is a biofluid cohort.
  • Blood, plasma, or serum cohort downgraded because this ranking targets tissue tumor-vs-normal comparisons.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

No dataset warnings

Normalized Fields

184 fields

Sample Group Preview

40 rows shown
Show Hide
Study totals: Case 30 | Control 10 | Unassigned 0 | Excluded 0 | Ambiguous 0 40 rows shown
Group Role Accession Title Source / characteristics Reason
Sepsis or systemic inflammatory response Case GSM8936500 Sepsis patient 1 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936501 Sepsis patient 2 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936502 Sepsis patient 3 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936503 Sepsis patient 4 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936504 Sepsis patient 5 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936505 Sepsis patient 6 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936506 Sepsis patient 7 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936507 Sepsis patient 8 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936508 Sepsis patient 9 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936509 Sepsis patient 10 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936510 Sepsis patient 11 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936511 Sepsis patient 12 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936512 Sepsis patient 13 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936513 Sepsis patient 14 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936514 Sepsis patient 15 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936515 Sepsis patient 16 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936516 Sepsis patient 17 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936517 Sepsis patient 18 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936518 Sepsis patient 19 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936519 Sepsis patient 20 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936520 Sepsis patient 21 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936521 Sepsis patient 22 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936522 Sepsis patient 23 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936523 Sepsis patient 24 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936524 Sepsis patient 25 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936525 Sepsis patient 26 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936526 Sepsis patient 27 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936527 Sepsis patient 28 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936528 Sepsis patient 29 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Sepsis or systemic inflammatory response Case GSM8936529 Sepsis patient 30 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Explicit sample label case marker detected.
Healthy control Control GSM8936490 Healthy control 1 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Sample-local control marker detected.
Healthy control Control GSM8936491 Healthy control 2 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Sample-local control marker detected.
Healthy control Control GSM8936492 Healthy control 3 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Sample-local control marker detected.
Healthy control Control GSM8936493 Healthy control 4 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Sample-local control marker detected.
Healthy control Control GSM8936494 Healthy control 5 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Sample-local control marker detected.
Healthy control Control GSM8936495 Healthy control 6 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Sample-local control marker detected.
Healthy control Control GSM8936496 Healthy control 7 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Sample-local control marker detected.
Healthy control Control GSM8936497 Healthy control 8 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Sample-local control marker detected.
Healthy control Control GSM8936498 Healthy control 9 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Sample-local control marker detected.
Healthy control Control GSM8936499 Healthy control 10 Whole blood cells Tissue: Whole blood cells; Cell type: Whole blood cells Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.