GSE296485

The RNA-seq of Ulcerative colitis patients and health

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

6 case / 3 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

The RNA extracted from left-sided colonic mucosal biopsy specimens of active UC patients (n=3), remission UC patients (n=3), and healthy controls (n=3) was subjected to RNA-seq.

Overall design

The RNA extracted from left-sided colonic mucosal biopsy specimens of active UC patients (n=3), remission UC patients (n=3), and healthy controls (n=3) was subjected to RNA-seq.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
9 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Publication context when PubMed identifiers are already known.

No match found

Metadata Quality Components

Analysis Readiness

Ready after review
100% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

10/10 pts

No dataset warnings are present.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Standard analyst review

Proceed only after the analyst confirms grouping, exclusions, and source files.

By Siftome standards, no expert review was triggered.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Disease context Ulcerative colitis Disease context came from Normalization rules.
  • Likely case group Ulcerative colitis 6 samples
  • Likely control group Healthy control 3 samples

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Biopsy. Comparison suitability: 0%.
  • Prioritized assay: bulk RNA-seq is the active assay expectation.
  • Interpreted disease metadata: Ulcerative colitis.
  • Processed data is available for downstream review.

Cautions

  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Overall score capped because fewer than ten samples limit cohort confidence.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

No dataset warnings

Normalized Fields

55 fields

Sample Group Preview

9 rows shown
Show Hide
Study totals: Case 6 | Control 3 | Unassigned 0 | Excluded 0 | Ambiguous 0 9 rows shown
Group Role Accession Title Source / characteristics Reason
Ulcerative colitis Case GSM8971235 Active ulcerative colitis patient-1 Left-sided colonic mucosal biopsy specimens Tissue: Left-sided colonic mucosal biopsy specimens; Genotype: UC Case marker detected.
Ulcerative colitis Case GSM8971236 Active ulcerative colitis patient-2 Left-sided colonic mucosal biopsy specimens Tissue: Left-sided colonic mucosal biopsy specimens; Genotype: UC Case marker detected.
Ulcerative colitis Case GSM8971237 Active ulcerative colitis patient-3 Left-sided colonic mucosal biopsy specimens Tissue: Left-sided colonic mucosal biopsy specimens; Genotype: UC Case marker detected.
Ulcerative colitis Case GSM8971238 Remission ulcerative colitis patient-1 Left-sided colonic mucosal biopsy specimens Tissue: Left-sided colonic mucosal biopsy specimens; Genotype: UC Case marker detected.
Ulcerative colitis Case GSM8971239 Remission ulcerative colitis patient-2 Left-sided colonic mucosal biopsy specimens Tissue: Left-sided colonic mucosal biopsy specimens; Genotype: UC Case marker detected.
Ulcerative colitis Case GSM8971240 Remission ulcerative colitis patient-3 Left-sided colonic mucosal biopsy specimens Tissue: Left-sided colonic mucosal biopsy specimens; Genotype: UC Case marker detected.
Healthy control Control GSM8971241 Healthy control-1 Left-sided colonic mucosal biopsy specimens Tissue: Left-sided colonic mucosal biopsy specimens; Genotype: HC Sample-local control marker detected.
Healthy control Control GSM8971242 Healthy control-2 Left-sided colonic mucosal biopsy specimens Tissue: Left-sided colonic mucosal biopsy specimens; Genotype: HC Sample-local control marker detected.
Healthy control Control GSM8971243 Healthy control-3 Left-sided colonic mucosal biopsy specimens Tissue: Left-sided colonic mucosal biopsy specimens; Genotype: HC Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.