GSE33943

Gene expression profiles of pediatric IBD remission patients

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

45 case / 13 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

Clinical remission is apparent when laboratory markers of inflammation are normal and clinical symptoms are absent. However, sub-clinical inflammation can still be present. A detailed analysis of the immune status during this inactive state of disease may provide a useful tool to subcategorize patients with subclinical immune activation We performed (un)supervised clustering analysis of IBD-associated genes and applied Ingenuity® pathway software to identify specific molecular profiles between patients.

Overall design

We analyzed RNA gene expression profiles of peripheral blood leucocytes (PBL) from pediatric IBD patients in clinical remission and age-matched controls.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
58 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

3 linked publications 3 DOIs

Primary publication

  1. Gene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission. PloS one, 2013

Related publications

  1. Blood transcriptomics identifies FEZ1 as a potential biomarker for inflammatory bowel disease. Computers in biology and medicine, 2025
  2. Identification of crucial genes of pediatric inflammatory bowel disease in remission by protein-protein interaction network and module analyses. Minerva pediatrics, 2023
Available

Metadata Quality Components

Analysis Readiness

Ready after review
97% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Inflammatory bowel disease Disease context came from Normalization rules.
  • Likely case group Inflammatory bowel disease 45 samples
  • Likely control group Control 13 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded for tissue search because it is a biofluid cohort. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Biofluid. Comparison suitability: 0%.
  • Interpreted disease metadata: Inflammatory bowel disease.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 3 publications; 3 DOIs available: PMID 24260248, PMID 29381010, PMID 39894009.
  • Publication context: Gene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission.. PloS one, 2013. PMID 24260248. DOI 10.1371/journal.pone.0079549.
  • Publication context: Identification of crucial genes of pediatric inflammatory bowel disease in remission by protein-protein interaction network and module analyses.. Minerva pediatrics, 2023. PMID 29381010. DOI 10.23736/S2724-5276.18.04997-6.
  • Publication context: Blood transcriptomics identifies FEZ1 as a potential biomarker for inflammatory bowel disease.. Computers in biology and medicine, 2025. PMID 39894009. DOI 10.1016/j.compbiomed.2025.109742.

Cautions

  • Downgraded for tissue search because it is a biofluid cohort.
  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Blood, plasma, or serum cohort downgraded because this ranking targets tissue tumor-vs-normal comparisons.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

107 fields

Sample Group Preview

58 rows shown
Show Hide
Study totals: Case 45 | Control 13 | Unassigned 0 | Excluded 0 | Ambiguous 0 58 rows shown
Group Role Accession Title Source / characteristics Reason
Inflammatory bowel disease Case GSM839186 Pieter_01 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839187 Pieter_02 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839188 Pieter_03 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839189 Pieter_4-2 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839190 Pieter_05 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839191 Pieter_06 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839192 Pieter_08 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839193 Pieter_10-2 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839194 Pieter_11 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839195 Pieter_13-2 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839196 Pieter_14-2 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839197 Pieter_17-2 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839198 Pieter_18-2 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839199 Pieter_19-2 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839200 Pieter_20 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839201 Pieter_21 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839202 Pieter_22 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839203 Pieter_24 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839204 Pieter_25 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839205 Pieter_26 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839206 Pieter_27 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839207 Pieter_28 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839208 Pieter_29 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839209 Pieter_30 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839210 Pieter_31 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839211 Pieter_32 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839212 Pieter_33 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839213 Pieter_34 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839214 Pieter_35 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839215 Pieter_36 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839216 Pieter_37 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839217 Pieter_38 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839218 Pieter_39 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839219 Pieter_40 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839220 Pieter_41 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839221 Pieter_42 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839222 Pieter_43 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839223 Pieter_44-2 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839224 Pieter_47-2 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839225 Pieter_48-2 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839226 Pieter_50 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839227 Pieter_51 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839228 Pieter_52 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839229 Pieter_53 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Inflammatory bowel disease Case GSM839230 Pieter_54 Pediatric IBD patients in clinical remission Tissue/cell type: Peripheral blood leucocytes (PBL) Case marker detected.
Control Control GSM839231 Pieter_101 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839232 Pieter_102 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839233 Pieter_104 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839234 Pieter_105 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839235 Pieter_106 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839236 Pieter_107 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839237 Pieter_108 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839238 Pieter_110 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839239 Pieter_111 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839240 Pieter_112 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839241 Pieter_113 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839242 Pieter_114 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.
Control Control GSM839243 Pieter_45 Age-matched control Tissue/cell type: Peripheral blood leucocytes (PBL) Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.