GSE50760

Gene expression profiling study by RNA-seq in colorectal cancer

Whole-study status Recommended

Scores and metadata are strong enough for direct review.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

36 case / 18 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

The objective of this study is to identify a prognostic signature in colorectal cancer (CRC) patients with diverse progression and heterogeneity of CRCs. We generated RNA-seq data of 54 samples (normal colon, primary CRC, and liver metastasis) from 18 CRC patients and, from the RNA-seq data, identified significant genes associated with aggressiveness of CRC. Through diverse statistical methods including generalized linear model likelihood ratio test, two significantly activated regulators were identified. In the validation cohorts, two activated regulators were independent risk factors and potential chemotherapy-sensitive agenets in colorectal cancers.

Overall design

RNA-seq data of 54 samples (normal colon, primary CRC, and liver metastasis) were generated from 18 CRC patients. Total RNA was isolated by RNeasy Mini Kit (Qiagen, CA, USA), according to the manufacturer's protocol. The quality and integrity of the RNA were confirmed by agarose gel electrophoresis and ethidium bromide staining, followed by visual examination under ultraviolet light. Sequencing library was prepared using TruSeq RNA Sample Preparation kit v2 (Illumina, CA, USA) according to the manufacturer’s protocols. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, fragmented, and converted into cDNAs. Then, adapters were ligated and the fragments were amplified on a PCR. Sequencing was performed in paired end reads (2x100 bp) using Hiseq-2000 (Illumina).

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
54 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Curated expression experiment linkage and source URL.

Expression Atlas accession
E-GEOD-50760
Available
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

19 linked publications 18 DOIs

Primary publication

  1. A nineteen gene-based risk score classifier predicts prognosis of colorectal cancer patients. Molecular oncology, 2014
  2. A prognostic index based on an eleven gene signature to predict systemic recurrences in colorectal cancer. Experimental & molecular medicine, 2019
  3. CILP2 is a potential biomarker for the prediction and therapeutic target of peritoneal metastases in colorectal cancer. Scientific reports, 2024
  4. Complex Behavior of ALDH1A1 and IGFBP1 in Liver Metastasis from a Colorectal Cancer. PloS one, 2016

Related publications

  1. Prognostic model development for classification of colorectal adenocarcinoma by using machine learning model based on feature selection technique boruta. Scientific reports, 2023
  2. Toward Colorectal Cancer Biomarkers: The Role of Genetic Variation, Wnt Pathway, and Long Noncoding RNAs. Omics : a journal of integrative biology, 2021
  3. Bioinformatics screening of colorectal-cancer causing molecular signatures through gene expression profiles to discover therapeutic targets and candidate agents. BMC medical genomics, 2023
  4. Genome-wide mutation profiles of colorectal tumors and associated liver metastases at the exome and transcriptome levels. Oncotarget, 2015
  5. FOXP3 promotes colorectal carcinoma liver metastases by evaluating MMP9 expression via regulating S-adenosylmethionine metabolism. Annals of translational medicine, 2020
  6. OTOP2, Inversely Modulated by miR-3148, Inhibits CRC Cell Migration, Proliferation and Epithelial-Mesenchymal Transition: Evidence from Bioinformatics Data Mining and Experimental Verification. Cancer management and research, 2022
  7. Reliable epithelial-mesenchymal transition biomarkers for colorectal cancer detection. Biomarkers in medicine, 2022
  8. Molecular insights into the development of hepatic metastases in colorectal cancer: a metastasis prediction study. European review for medical and pharmacological sciences, 2020
  9. Effect of ISM1 on the Immune Microenvironment and Epithelial-Mesenchymal Transition in Colorectal Cancer. Frontiers in cell and developmental biology, 2021
  10. Identification of key claudin genes associated with survival prognosis and diagnosis in colon cancer through integrated bioinformatic analysis. Frontiers in genetics, 2023
  11. Unlocking the Potential of the CA2, CA7, and ITM2C Gene Signatures for the Early Detection of Colorectal Cancer: A Comprehensive Analysis of RNA-Seq Data by Utilizing Machine Learning Algorithms. Genes, 2023
  12. Colorectal Cancer Biomarker Identification via Joint DNA-Methylation and Transcriptomics Analysis Workflow. Genes, 2025
  13. Sp1 contributes to overexpression of stanniocalcin 2 through regulation of promoter activity in colon adenocarcinoma. World journal of gastroenterology, 2019
  14. PCK1 and DHODH drive colorectal cancer liver metastatic colonization and hypoxic growth by promoting nucleotide synthesis. eLife, 2019
  15. ARNTL2 knockdown suppressed the invasion and migration of colon carcinoma: decreased SMOC2-EMT expression through inactivation of PI3K/AKT pathway. American journal of translational research, 2020
Available

Metadata Quality Components

Analysis Readiness

Ready after review
100% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

10/10 pts

No dataset warnings are present.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Standard analyst review

Proceed only after the analyst confirms grouping, exclusions, and source files.

By Siftome standards, no expert review was triggered.

Why This Is Ranked Here

Tumor vs normal
Recommended Scores and metadata are strong enough for direct review.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Disease context Colorectal cancer Disease context came from Normalization rules.
  • Likely case group Colorectal cancer 36 samples
  • Likely control group Normal tissue 18 samples

Ranking Facts

Supporting evidence

  • Comparison: Tumor vs unmatched normal. Specimen: Tissue. Comparison suitability: 100%.
  • Prioritized assay: bulk RNA-seq is the active assay expectation.
  • Interpreted disease metadata: Colorectal cancer.
  • Processed data is available for downstream review.
  • External evidence: Expression Atlas curated experiment link is available.
  • External evidence: PubMed links 19 publications; 18 DOIs available: PMID 25049118, PMID 26109429, PMID 27152521, PMID 31236000, PMID 31578316.
  • Publication context: A nineteen gene-based risk score classifier predicts prognosis of colorectal cancer patients.. Molecular oncology, 2014. PMID 25049118. DOI 10.1016/j.molonc.2014.06.016.
  • Publication context: Genome-wide mutation profiles of colorectal tumors and associated liver metastases at the exome and transcriptome levels.. Oncotarget, 2015. PMID 26109429. DOI 10.18632/oncotarget.4246.
  • Publication context: Complex Behavior of ALDH1A1 and IGFBP1 in Liver Metastasis from a Colorectal Cancer.. PloS one, 2016. PMID 27152521. DOI 10.1371/journal.pone.0155160.
  • Publication context: 16 additional linked PubMed publications are available.

Dataset Warnings

No dataset warnings

Normalized Fields

311 fields

Sample Group Preview

54 rows shown
Show Hide
Study totals: Case 36 | Control 18 | Unassigned 0 | Excluded 0 | Ambiguous 0 54 rows shown
Group Role Accession Title Source / characteristics Reason
Colorectal cancer Case GSM1228184 Primary colorectal cancer AMC_2-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228185 Primary colorectal cancer AMC_3-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228186 Primary colorectal cancer AMC_5-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228187 Primary colorectal cancer AMC_6-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228188 Primary colorectal cancer AMC_7-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228189 Primary colorectal cancer AMC_8-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228190 Primary colorectal cancer AMC_9-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228191 Primary colorectal cancer AMC_10-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228192 Primary colorectal cancer AMC_12-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228193 Primary colorectal cancer AMC_13-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228194 Primary colorectal cancer AMC_17-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228195 Primary colorectal cancer AMC_18-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228196 Primary colorectal cancer AMC_19-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228197 Primary colorectal cancer AMC_20-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228198 Primary colorectal cancer AMC_21-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228199 Primary colorectal cancer AMC_22-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228200 Primary colorectal cancer AMC_23-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Colorectal cancer Case GSM1228201 Primary colorectal cancer AMC_24-1 Primary colorectal cancer Tissue: Primary colorectal cancer; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228220 Metastasized cancer AMC_2-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228221 Metastasized cancer AMC_3-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228222 Metastasized cancer AMC_5-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228223 Metastasized cancer AMC_6-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228224 Metastasized cancer AMC_7-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228225 Metastasized cancer AMC_8-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228226 Metastasized cancer AMC_9-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228227 Metastasized cancer AMC_10-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228228 Metastasized cancer AMC_12-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228229 Metastasized cancer AMC_13-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228230 Metastasized cancer AMC_17-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228231 Metastasized cancer AMC_18-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228232 Metastasized cancer AMC_19-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228233 Metastasized cancer AMC_20-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228234 Metastasized cancer AMC_21-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228235 Metastasized cancer AMC_22-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228236 Metastasized cancer AMC_23-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Tumor sample Case GSM1228237 Metastasized cancer AMC_24-3 Metastasized cancer Tissue: Metastatic colorectal cancer to the liver; Ajcc stage: Stage IV Case marker detected.
Normal tissue Control GSM1228202 Normal colon AMC_2-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228203 Normal colon AMC_3-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228204 Normal colon AMC_5-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228205 Normal colon AMC_6-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228206 Normal colon AMC_7-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228207 Normal colon AMC_8-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228208 Normal colon AMC_9-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228209 Normal colon AMC_10-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228210 Normal colon AMC_12-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228211 Normal colon AMC_13-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228212 Normal colon AMC_17-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228213 Normal colon AMC_18-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228214 Normal colon AMC_19-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228215 Normal colon AMC_20-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228216 Normal colon AMC_21-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228217 Normal colon AMC_22-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228218 Normal colon AMC_23-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.
Normal tissue Control GSM1228219 Normal colon AMC_24-2 Normal colon Tissue: Normal-looking surrounding colonic epithelium; Ajcc stage: Stage IV Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.