GSE53306

Differential Gene Expression between Active and Quiescent Stages of Ulcerative Colitis: Insights into a Distinctive Pathogenesis Profile

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

28 case / 12 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

Our objective was to elucidate patterns of gene expression underlying the progression of UC disease. Single endoscopic pinch biopsies (n=40) were assayed using the Illumina cDNA-mediated Annealing, Selection, Extension, and Ligation (DASL) microarray. Tissue was sampled in consecutive active and quiescent stages at the same site in individual UC patients, and these stages (active and inactive) were compared with each other as well as to non-inflammatory bowel disease (non-IBD) healthy controls.

Overall design

Gene expression of UC active and inactive is compared at a global level using total RNA from the same UC pateints using the Illumina microarray platform.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
40 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

10 linked publications 10 DOIs

Primary publication

  1. Mobilization of epithelial mesenchymal transition genes distinguishes active from inactive lesional tissue in patients with ulcerative colitis. Human molecular genetics, 2015

Related publications

  1. Identification and Validation of the Diagnostic Markers for Inflammatory Bowel Disease by Bioinformatics Analysis and Machine Learning. Biochemical genetics, 2024
  2. Identification and validation of GNA15 as a novel diagnostic biomarker in ulcerative colitis. Journal of molecular histology, 2026
  3. Exploring Pyroptosis-related Signature Genes and Potential Drugs in Ulcerative Colitis by Transcriptome Data and Animal Experimental Validation. Inflammation, 2024
  4. Identification of useful genes from multiple microarrays for ulcerative colitis diagnosis based on machine learning methods. Scientific reports, 2022
  5. Identification of immune-related hub genes contributing to the pathogenesis, diagnosis, and remission of ulcerative colitis by integrated bioinformatic analyses. Medicine, 2023
  6. Supplementation of Glucosamine Selenium Ameliorates DSS-Induced Chronic Colitis in Mice via Affecting Gut Microbiota, Inhibiting Pyroptosis and Inactivating Chemokine Signaling Pathway. Journal of inflammation research, 2025
  7. Analysis of cuproptosis-related genes in Ulcerative colitis and immunological characterization based on machine learning. Frontiers in medicine, 2023
  8. Identification of Pharmacological Autophagy Regulators of Active Ulcerative Colitis. Frontiers in pharmacology, 2021
  9. Integrating Transcriptomics and Machine Learning to Uncover the FLI1-PARP14-Immune Axis in Ulcerative Colitis Activity and Pathogenesis. Genes, 2025
Available

Metadata Quality Components

Analysis Readiness

Ready after review
97% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Inflammatory bowel disease, ulcerative colitis Disease context came from Normalization rules.
  • Likely case group Ulcerative colitis 28 samples
  • Likely control group Healthy control 12 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded assay: microarray cohorts are separated from the RNA-seq intent. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Tissue. Comparison suitability: 0%.
  • Interpreted disease metadata: Inflammatory bowel disease, Ulcerative colitis.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 10 publications; 10 DOIs available: PMID 26034135, PMID 34925026, PMID 35705632, PMID 37351719, PMID 37529244.
  • Publication context: Mobilization of epithelial mesenchymal transition genes distinguishes active from inactive lesional tissue in patients with ulcerative colitis.. Human molecular genetics, 2015. PMID 26034135. DOI 10.1093/hmg/ddv192.
  • Publication context: Identification of Pharmacological Autophagy Regulators of Active Ulcerative Colitis.. Frontiers in pharmacology, 2021. PMID 34925026. DOI 10.3389/fphar.2021.769718.
  • Publication context: Identification of useful genes from multiple microarrays for ulcerative colitis diagnosis based on machine learning methods.. Scientific reports, 2022. PMID 35705632. DOI 10.1038/s41598-022-14048-6.
  • Publication context: 7 additional linked PubMed publications are available.

Cautions

  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Microarray expression context downgraded below primary bulk RNA-seq for the default tissue ranking.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Overall score capped because microarray cohorts should be compared separately from RNA-seq cohorts.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

148 fields

Sample Group Preview

40 rows shown
Show Hide
Study totals: Case 28 | Control 12 | Unassigned 0 | Excluded 0 | Ambiguous 0 40 rows shown
Group Role Accession Title Source / characteristics Reason
Ulcerative colitis Case GSM1289168 UC_active_1 Colon tissue Patient: UC patient #1; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289169 UC_active_2 Colon tissue Patient: UC patient #2; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289170 UC_active_4 Colon tissue Patient: UC patient #4; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289171 UC_active_5 Colon tissue Patient: UC patient #5; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289172 UC_active_6 Colon tissue Patient: UC patient #6; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289173 UC_active_7 Colon tissue Patient: UC patient #7; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289174 UC_active_8 Colon tissue Patient: UC patient #8; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289175 UC_active_9 Colon tissue Patient: UC patient #9; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289176 UC_active_10 Colon tissue Patient: UC patient #10; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289177 UC_active_11-1 Colon tissue Patient: UC patient #11; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289178 UC_active_11-2 Colon tissue Patient: UC patient #11 (same site, post-relapse); Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289179 UC_active_12-1 Colon tissue Patient: UC patient #12; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289180 UC_active_12-2 Colon tissue Patient: UC patient #12 (same site, post-relapse); Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289181 UC_active_13-1 Colon tissue Patient: UC patient #13; Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289182 UC_active_13-2 Colon tissue Patient: UC patient #13 (same site, post-relapse); Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289183 UC_active_13-3 Colon tissue Patient: UC patient #13 (same site, post-relapse2); Disease state: Ulcerative colitis active Case marker detected.
Ulcerative colitis Case GSM1289184 UC_inactive_1 Colon tissue Patient: UC patient #1 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289185 UC_inactive_2 Colon tissue Patient: UC patient #2 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289186 UC_inactive_4 Colon tissue Patient: UC patient #4 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289187 UC_inactive_5 Colon tissue Patient: UC patient #5 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289188 UC_inactive_6 Colon tissue Patient: UC patient #6 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289189 UC_inactive_7 Colon tissue Patient: UC patient #7 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289190 UC_inactive_8 Colon tissue Patient: UC patient #8 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289191 UC_inactive_9 Colon tissue Patient: UC patient #9 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289192 UC_inactive_10 Colon tissue Patient: UC patient #10 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289193 UC_inactive_11 Colon tissue Patient: UC patient #11 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289194 UC_inactive_13-1 Colon tissue Patient: UC patient #13 (same site, remission); Disease state: Ulcerative colitis inactive Case marker detected.
Ulcerative colitis Case GSM1289195 UC_inactive_13-2 Colon tissue Patient: UC patient #13 (same site, remission2); Disease state: Ulcerative colitis inactive Case marker detected.
Healthy control Control GSM1289196 Normal_control_1 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289197 Normal_control_2 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289198 Normal_control_3 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289199 Normal_control_4 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289200 Normal_control_5 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289201 Normal_control_6 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289202 Normal_control_7 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289203 Normal_control_8 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289204 Normal_control_9 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289205 Normal_control_10 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289206 Normal_control_11 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.
Healthy control Control GSM1289207 Normal_control_12 Colon tissue Tissue: Colon; Disease state: Healthy control Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.