GSE66208

mRNA and small RNA associated with Crohn's disease behavior [ncRNA-Seq]

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

27 case / 14 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

There is a need for reliable prognostic markers that can guide therapeutic intervention in Crohn’s disease (CD). We examined whether different behavioral phenotypes in CD can be classified based on colonic miRNA or mRNA expression and if miRNAs have prognostic utility for CD. We perform high-throughput sequencing of small RNA and mRNA isolated from colon tissue from CD patients and non-IBD (NIBD) controls. To identify miRNA and genes associated with specific behavioral phenotypes of CD, patients were stratified according to disease behavior (non-stricturing, non-penetrating; stricturing; penetrating) and compared miRNA profiles in each class with those of the NIBD group. Using a novel statistical simulation approach applied to colonic RNA-seq data for CD patients and NIBD controls, we identify at drivers of gene expression profiles associated with CD.

Overall design

Macroscopically non-inflamed colon tissue from characterized Crohn's disease patients and normal controls were obtained. Small RNA-seq and RNA-seq were performed on these samples. Additionally, we investigated the effect of inflammation on miRNA expression by performing small RNA-seq on matched colon samples obtained from macroscopically inflamed regions from a subset (six) of these patients with CD.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
41 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

1 linked publication 1 DOI

Primary publication

  1. MicroRNAs Classify Different Disease Behavior Phenotypes of Crohn's Disease and May Have Prognostic Utility. Inflammatory bowel diseases, 2015
Available

Metadata Quality Components

Analysis Readiness

Review before analysis
97% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

  • Secondary RNA assay context should be analyzed separately from ordinary bulk RNA-seq.

Next Steps

  • Review blockers and update sample grouping or dataset selection before analysis.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Analysis readiness has blockers that must be resolved before DE.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Disease context Crohn's disease, colorectal cancer, inflammatory bowel disease Disease context came from Normalization rules.
  • Likely case group Inflamed tissue 27 samples
  • Likely control group Mixed controls 14 samples

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Biopsy. Comparison suitability: 0%.
  • Prioritized assay: bulk RNA-seq is the active assay expectation.
  • Interpreted disease metadata: Colorectal cancer, Crohn's disease, Inflammatory bowel disease.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 1 publication; 1 DOI available: PMID 26164662.
  • Publication context: MicroRNAs Classify Different Disease Behavior Phenotypes of Crohn's Disease and May Have Prognostic Utility.. Inflammatory bowel diseases, 2015. PMID 26164662. DOI 10.1097/MIB.0000000000000478.

Cautions

  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Small RNA, miRNA, circRNA, or extracellular-vesicle RNA context downgraded for primary mRNA tissue ranking.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Overall score capped because secondary RNA assays are not primary mRNA tissue RNA-seq.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

185 fields

Sample Group Preview

41 rows shown
Show Hide
Study totals: Case 27 | Control 14 | Unassigned 0 | Excluded 0 | Ambiguous 0 41 rows shown
Group Role Accession Title Source / characteristics Reason
Inflamed tissue Case GSM1616959 B2/stricturing Crohn's disease [ncRNA-Seq, 20_CD_NI_B2] Colon Age: 15; Sex: Male; Disease: Crohn's Disease; Disease_stage: B2/stricturing; Inflamed: No; Montreal classification: A1L1B2 Case marker detected.
Inflamed tissue Case GSM1616960 B2/stricturing Crohn's disease [ncRNA-Seq, 21_CD_NI_B2] Colon Age: 51; Sex: Female; Disease: Crohn's Disease; Disease_stage: B2/stricturing; Inflamed: No; Montreal classification: A2L2B2 Case marker detected.
Inflamed tissue Case GSM1616961 B3/penetrating Crohn's disease [ncRNA-Seq, 29_CD_NI_B3] Colon Age: 59; Sex: Female; Disease: Crohn's Disease; Disease_stage: B3/penetrating; Inflamed: No; Montreal classification: A2L3B3 Case marker detected.
Inflamed tissue Case GSM1616962 B1/non-strituring, non-penetrating Crohn's disease [ncRNA-Seq, 405_CD_NI_B1] Colon Age: 56; Sex: Female; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: No; Montreal classification: A2L2B1 Case marker detected.
Inflamed tissue Case GSM1616963 B3/penetrating Crohn's disease [ncRNA-Seq, 407_CD_NI_B3] Colon Age: 43; Sex: Female; Disease: Crohn's Disease; Disease_stage: B3/penetrating; Inflamed: No; Montreal classification: A2L2B3p Case marker detected.
Inflamed tissue Case GSM1616964 B1/non-strituring, non-penetrating Crohn's disease [ncRNA-Seq, 408_CD_NI_B1] Colon Age: 23; Sex: Female; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: No; Montreal classification: A2L2B1 Case marker detected.
Inflamed tissue Case GSM1616965 B1/non-strituring, non-penetrating Crohn's disease [ncRNA-Seq, 413_CD_NI_B1] Colon Age: 32; Sex: Female; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: No; Montreal classification: A2L2B1 Case marker detected.
Inflamed tissue Case GSM1616966 B2/stricturing Crohn's disease [ncRNA-Seq, 420_CD_NI_B2] Colon Age: 37; Sex: Female; Disease: Crohn's Disease; Disease_stage: B2/stricturing; Inflamed: No; Montreal classification: A2L2B2p Case marker detected.
Inflamed tissue Case GSM1616967 B2/stricturing Crohn's disease [ncRNA-Seq, 422_CD_NI_B2] Colon Age: 21; Sex: Female; Disease: Crohn's Disease; Disease_stage: B2/stricturing; Inflamed: No; Montreal classification: A1L3B2 Case marker detected.
Inflamed tissue Case GSM1616968 B3/penetrating Crohn's disease [ncRNA-Seq, 424_CD_NI_B3] Colon Age: 34; Sex: Male; Disease: Crohn's Disease; Disease_stage: B3/penetrating; Inflamed: No; Montreal classification: A2L3B3 Case marker detected.
Inflamed tissue Case GSM1616969 B1/non-strituring, non-penetrating Crohn's disease [ncRNA-Seq, 429_CD_NI_B1] Colon Age: 25; Sex: Female; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: No; Montreal classification: A2L3B1 Case marker detected.
Inflamed tissue Case GSM1616970 B1/non-strituring, non-penetrating Crohn's disease [ncRNA-Seq, 431_CD_NI_B1] Colon Age: 20; Sex: Female; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: No; Montreal classification: A1L2B1 Case marker detected.
Inflamed tissue Case GSM1616971 B1/non-strituring, non-penetrating Crohn's disease [ncRNA-Seq, 433_CD_NI_B1] Colon Age: 29; Sex: Male; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: No; Montreal classification: A2L3B1 Case marker detected.
Inflamed tissue Case GSM1616972 B3/penetrating Crohn's disease [ncRNA-Seq, 434_CD_NI_B3] Colon Age: 31; Sex: Male; Disease: Crohn's Disease; Disease_stage: B3/penetrating; Inflamed: No; Montreal classification: A2L3B3 Case marker detected.
Inflamed tissue Case GSM1616973 B2/stricturing Crohn's disease [ncRNA-Seq, 440_CD_NI_B2] Colon Age: 76; Sex: Male; Disease: Crohn's Disease; Disease_stage: B2/stricturing; Inflamed: No; Montreal classification: A2L3B2 Case marker detected.
Inflamed tissue Case GSM1616974 B3/penetrating Crohn's disease [ncRNA-Seq, 450_CD_NI_B3] Colon Age: 28; Sex: Female; Disease: Crohn's Disease; Disease_stage: B3/penetrating; Inflamed: No; Montreal classification: A2L1B3 Case marker detected.
Inflamed tissue Case GSM1616975 B2/stricturing Crohn's disease [ncRNA-Seq, 51_CD_NI_B2] Colon Age: 30; Sex: Female; Disease: Crohn's Disease; Disease_stage: B2/stricturing; Inflamed: No; Montreal classification: A2L3B2 Case marker detected.
Inflamed tissue Case GSM1616976 B1/non-strituring, non-penetrating Crohn's disease [ncRNA-Seq, 54_CD_NI_B1] Colon Age: 20; Sex: Male; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: No; Montreal classification: A1L2B1 Case marker detected.
Inflamed tissue Case GSM1616977 B3/penetrating Crohn's disease [ncRNA-Seq, 62_CD_NI_B3] Colon Age: 45; Sex: Female; Disease: Crohn's Disease; Disease_stage: B3/penetrating; Inflamed: No; Montreal classification: A2L3B3 Case marker detected.
Inflamed tissue Case GSM1616978 B1/non-strituring, non-penetrating Crohn's disease [ncRNA-Seq, 63_CD_NI_B1] Colon Age: 49; Sex: Male; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: No; Montreal classification: A1L3B1 Case marker detected.
Inflamed tissue Case GSM1616979 B3/penetrating Crohn's disease [ncRNA-Seq, 64_CD_NI_B3] Colon Age: 49; Sex: Female; Disease: Crohn's Disease; Disease_stage: B3/penetrating; Inflamed: No; Montreal classification: A2L3B3 Case marker detected.
Inflamed tissue Case GSM1616980 Inflamed region of patient with B3/penetrating Crohn's disease [ncRNA-Seq, 407_CD_IF_B3] Colon Age: 43; Sex: Female; Disease: Crohn's Disease; Disease_stage: B3/penetrating; Inflamed: Yes; Montreal classification: A2L2B3p Case marker detected.
Inflamed tissue Case GSM1616981 Inflamed region of patient with B1/non-stricturing, non-penetrating Crohn's disease [ncRNA-Seq, 408_CD_IF_B1] Colon Age: 23; Sex: Female; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: Yes; Montreal classification: A2L2B1 Case marker detected.
Inflamed tissue Case GSM1616982 Inflamed region of patient with B1/non-stricturing, non-penetrating Crohn's disease [ncRNA-Seq, 413_CD_IF_B1] Colon Age: 32; Sex: Female; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: Yes; Montreal classification: A2L2B1 Case marker detected.
Inflamed tissue Case GSM1616983 Inflamed region of patient with B1/non-stricturing, non-penetrating Crohn's disease [ncRNA-Seq, 431_CD_IF_B1] Colon Age: 20; Sex: Female; Disease: Crohn's Disease; Disease_stage: B1/non-strictuing, non-penetrating; Inflamed: Yes; Montreal classification: A1L2B1 Case marker detected.
Inflamed tissue Case GSM1616984 Inflamed region of patient with B3/penetrating Crohn's disease [ncRNA-Seq, 434_CD_IF_B3] Colon Age: 31; Sex: Male; Disease: Crohn's Disease; Disease_stage: B3/penetrating; Inflamed: Yes; Montreal classification: A2L3B3 Case marker detected.
Inflamed tissue Case GSM1616985 Inflamed region of patient with B2/stricturing Crohn's disease [ncRNA-Seq, 440_CD_IF_B2] Colon Age: 76; Sex: Male; Disease: Crohn's Disease; Disease_stage: B2/stricturing; Inflamed: Yes; Montreal classification: A2L3B2 Case marker detected.
Normal tissue Control GSM1616945 Normal colon biopsy, female age 53 [ncRNA-Seq, 22_NIBD_NI] Colon Age: 53; Sex: Female; Disease: Normal; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616946 Normal colon acquired during Hartmann reversal after colon cancer, male age 52 [ncRNA-Seq, 23_NIBD_NI] Colon Age: 52; Sex: Male; Disease: Normal, Hartmann reversal after colon cancer; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616947 Normal colon biopsy acquired during surgery for diverticulitis,female age 60 [ncRNA-Seq, 25_NIBD_NI] Colon Age: 60; Sex: Female; Disease: Normal, diverticulitis; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616948 Normal colon acquired during surgery for diverticulitis, male age 70 [ncRNA-Seq, 27_NIBD_NI] Colon Age: 70; Sex: Male; Disease: Normal, diverticulitis; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616949 Normal colon biopsy acquired during surgery for colon cancer, female age 82 [ncRNA-Seq, 30_NIBD_NI] Colon Age: 82; Sex: Female; Disease: Normal, Colon cancer; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616950 Normal colon biopsy acquired during surgery for colon cancer, male age 44 [ncRNA-Seq, 31_NIBD_NI] Colon Age: 44; Sex: Male; Disease: Normal, Colon cancer; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616951 Normal colon acquired during surgery for colon cancer, male age 44 [ncRNA-Seq, 32_NIBD_NI] Colon Age: 44; Sex: Male; Disease: Normal, Colon cancer; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616952 Normal colon acquired during surgery for colonic inertia, female age 41 [ncRNA-Seq, 36_NIBD_NI] Colon Age: 41; Sex: Female; Disease: Normal, colonic inertia, TI submucosal hemorrhage (but looked inflamed on macro); Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616953 Normal colon acquired during surgery for colon cancer, male age 52 [ncRNA-Seq, 39_NIBD_NI] Colon Age: 52; Sex: Male; Disease: Normal, Colon cancer; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Healthy control Control GSM1616954 Normal colon acquired during surgery for large polyp, male age 65 [ncRNA-Seq, 42_NIBD_NI] Colon Age: 65; Sex: Male; Disease: Normal control, large polyp; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616955 Normal colon acquired during surgery for colonic inertia, female age 45 [ncRNA-Seq, 43_NIBD_NI] Colon Age: 45; Sex: Female; Disease: Normal, colonic inertia; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616956 Normal colon acquired during surgery for adenoma, male age 70 [ncRNA-Seq, 48_NIBD_NI] Colon Age: 70; Sex: Male; Disease: Normal, Adenoma; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616957 Normal colon acquired during surgery for adenoma, male age 62 [ncRNA-Seq, 49_NIBD_NI] Colon Age: 62; Sex: Male; Disease: Normal, Adenoma; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.
Normal tissue Control GSM1616958 Normal colon acquired during surgery for small intestine neuroendocrine tumor, female age 49 [ncRNA-Seq, 50_NIBD_NI] Colon Age: 49; Sex: Female; Disease: Normal, SI Neuroendocrine tumor; Disease_stage: NA; Inflamed: No; Montreal classification: NA Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.