GSE6731

Genome-wide gene expression differences between Crohn’s and ulcerative colitis from endoscopic pinch biopsies:

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

28 case / 4 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

Ulcerative colitis (UC) and Crohn’s disease (CD) are inflammatory bowel diseases (IBD) with variable, overlapping clinical features and complex pathophysiologies. To identify pathogenic processes underlying these disease subtypes, using single endoscopic pinch biopsies to estabolish 36 expression profiles, we elucidated gene expression patterns of active and inactive areas of UC and CD, and compared these to infectious colitis and healthy controls. Keywords: RNA

Overall design

To identify pathogenic processes underlying these disease subtypes, using single endoscopic pinch biopsies, we elucidated gene expression patterns of active and inactive areas of UC and CD, and compared these to infectious colitis and healthy controls. An unsupervised classification of a total of 36 samples yielded promising separation between IBD affected, unaffected, non-IBD colitis and normal controls, suggesting distinctive gene expression patterns for each sample type. The Significance Analysis of Microarays (SAM) software to select biologically significant changes in gene expression between groups. The criteria selected for SAM analysis are, a median false discovery rate (FDR) ≤ 0.00001%, fold change >2, and a Log2 mean expression index > 6.64.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
36 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Curated expression experiment linkage and source URL.

Expression Atlas accession
E-GEOD-6731
Available
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

4 linked publications 3 DOIs

Primary publication

  1. Genome-wide gene expression differences in Crohn's disease and ulcerative colitis from endoscopic pinch biopsies: insights into distinctive pathogenesis. Inflammatory bowel diseases, 2007

Related publications

  1. Identification of useful genes from multiple microarrays for ulcerative colitis diagnosis based on machine learning methods. Scientific reports, 2022
  2. Identifying hub genes and miRNAs in Crohn's disease by bioinformatics analysis. Frontiers in genetics, 2022
  3. Bioinformatic analysis of potential candidates for therapy of inflammatory bowel disease. European review for medical and pharmacological sciences, 2015
Available

Metadata Quality Components

Analysis Readiness

Review before analysis
85% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

3/15 pts

Unassigned or review-needed samples remain before analysis.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

  • 4 samples are still unassigned.

Next Steps

  • Resolve unassigned or review-needed samples in the grouping table.

Analysis Preparation Checklist

4/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Needs review

0 review-needed samples and 4 unassigned samples remain.

Resolve ambiguous and unassigned samples before finalizing the contrast.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Analysis readiness has blockers that must be resolved before DE.

One or more samples are unassigned and need manual grouping decisions.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Crohn's disease, inflammatory bowel disease, ulcerative colitis Disease context came from Normalization rules.
  • Likely case group Crohn's disease 28 samples
  • Likely control group Normal tissue 4 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded assay: microarray cohorts are separated from the RNA-seq intent. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Biopsy. Comparison suitability: 0%.
  • Interpreted disease metadata: Crohn's disease, Infectious disease or host response, Inflammatory bowel disease, Ulcerative colitis.
  • Processed data is available for downstream review.
  • External evidence: Expression Atlas curated experiment link is available.
  • External evidence: PubMed links 4 publications; 3 DOIs available: PMID 17262812, PMID 26636514, PMID 35705632, PMID 36118873.
  • Publication context: Genome-wide gene expression differences in Crohn's disease and ulcerative colitis from endoscopic pinch biopsies: insights into distinctive pathogenesis.. Inflammatory bowel diseases, 2007. PMID 17262812. DOI 10.1002/ibd.20110.
  • Publication context: Bioinformatic analysis of potential candidates for therapy of inflammatory bowel disease.. European review for medical and pharmacological sciences, 2015. PMID 26636514.
  • Publication context: Identification of useful genes from multiple microarrays for ulcerative colitis diagnosis based on machine learning methods.. Scientific reports, 2022. PMID 35705632. DOI 10.1038/s41598-022-14048-6.
  • Publication context: 1 additional linked PubMed publications are available.

Cautions

  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

177 fields

Sample Group Preview

36 rows shown
Show Hide
Study totals: Case 28 | Control 4 | Unassigned 4 | Excluded 0 | Ambiguous 0 36 rows shown
Group Role Accession Title Source / characteristics Reason
Crohn's disease Case GSM155568 CD-33-Aff Colonoscopic biopsy from adult with affected colon with Crohn's disease Disease affected colon of an adult with Crohn's disease (CD). Case marker detected.
Crohn's disease Case GSM155569 CD-49-Aff1 Colonoscopic biopsy from adult with affected colon with Crohn's disease Disease affected colon of an adult with Crohn's disease (CD). Case marker detected.
Crohn's disease Case GSM155570 CD-51-Aff Colonoscopic biopsy from adult with affected colon with Crohn's disease Disease affected colon of an adult with Crohn's disease (CD). Case marker detected.
Crohn's disease Case GSM155571 CD-53-Aff Colonoscopic biopsy from adult with affected colon with Crohn's disease Disease affected colon of an adult with Crohn's disease (CD). Case marker detected.
Crohn's disease Case GSM155572 CD-58-Aff Colonoscopic biopsy from adult with affected colon with Crohn's disease Disease affected colon of an adult with Crohn's disease (CD). Case marker detected.
Crohn's disease Case GSM155573 CD-76-Aff1 Colonoscopic biopsy from adult with affected colon with Crohn's disease Disease affected colon of an adult with Crohn's disease (CD). Replicate-1 Case marker detected.
Crohn's disease Case GSM155574 CD-76-Aff2 Colonoscopic biopsy from adult with affected colon with Crohn's disease Disease affected colon of an adult with Crohn's disease (CD). Replicate-2 Case marker detected.
Crohn's disease Case GSM155575 CD-33-un Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155576 CD-45-un Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155577 CD-45-un2 Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155578 CD-48-un Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155579 CD-48-un2 Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155580 CD-49-un1 Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155581 CD-51-un Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155582 CD-53-un Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155583 CD-58-un Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155584 CD-59-un Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155585 CD-59-un2 Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Crohn's disease Case GSM155586 CD-76-un Colonoscopic biopsy from adult with unaffected colon with Crohn's disease Disease unaffected colon of a adult with Crohn's disease (CD) Case marker detected.
Ulcerative colitis Case GSM155587 UC-32-Aff Colonoscopic biopsy from adult with affected colon from adult with UC Disease affected colon of an adult with ulcerative colitis (UC). Case marker detected.
Ulcerative colitis Case GSM155588 UC-35-Aff Colonoscopic biopsy from adult with affected colon from adult with UC Disease affected colon of an adult with ulcerative colitis (UC). Case marker detected.
Ulcerative colitis Case GSM155589 UC-38-Aff Colonoscopic biopsy from adult with affected colon from adult with UC Disease affected colon of an adult with ulcerative colitis (UC). Case marker detected.
Ulcerative colitis Case GSM155590 UC-55-Aff Colonoscopic biopsy from adult with affected colon from adult with UC Disease affected colon of an adult with ulcerative colitis (UC). Case marker detected.
Ulcerative colitis Case GSM155591 UC-71-Aff Colonoscopic biopsy from adult with affected colon from adult with UC Disease affected colon of an adult with ulcerative colitis (UC). Case marker detected.
Ulcerative colitis Case GSM155592 UC-32-un Colonoscopic biopsy from adult with unaffected colon from adult with UC Disease unaffected colon of a adult with ulcerative colitis (UC). Case marker detected.
Ulcerative colitis Case GSM155593 UC-33-un Colonoscopic biopsy from adult with unaffected colon from adult with UC Disease unaffected colon of a adult with ulcerative colitis (UC). Case marker detected.
Ulcerative colitis Case GSM155594 UC-38-un Colonoscopic biopsy from adult with unaffected colon from adult with UC Disease unaffected colon of a adult with ulcerative colitis (UC). Case marker detected.
Ulcerative colitis Case GSM155595 UC-55-un Colonoscopic biopsy from adult with unaffected colon from adult with UC Disease unaffected colon of a adult with ulcerative colitis (UC). Case marker detected.
Normal tissue Control GSM155564 N65s2 Colonoscopic biopsy from normal adult Normal sigmoid colon of adult Control marker detected.
Normal tissue Control GSM155565 N66s1 Colonoscopic biopsy from normal adult Normal sigmoid colon of adult Control marker detected.
Normal tissue Control GSM155566 N69s Colonoscopic biopsy from normal adult Normal sigmoid colon of adult Control marker detected.
Normal tissue Control GSM155567 N79s Colonoscopic biopsy from normal adult Normal sigmoid colon of adult Control marker detected.
Unassigned Unassigned GSM155596 INF-156 Colonoscopic biopsy from adult with affected colon from adult with INF Disease affected colon of an adult with bacterial infectious colitis (INF). No explicit case or control marker detected.
Unassigned Unassigned GSM155597 INF-157 Colonoscopic biopsy from adult with affected colon from adult with INF Disease affected colon of an adult with bacterial infectious colitis (INF). No explicit case or control marker detected.
Unassigned Unassigned GSM155598 IC-44-Aff Colonoscopic biopsy from adult with affected colon from adult with IC Disease affected colon of an adult with indeterminate colitis (IC). No explicit case or control marker detected.
Unassigned Unassigned GSM155599 IC-44-un Colonoscopic biopsy from adult with unaffected colon from adult with IC Disease unaffected colon of an adult with indeterminate colitis (IC). No explicit case or control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.