GSE74265

Gene expression signature and mitochondrial DNA copy number variation in ulcerative colitis patients

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

30 case / 0 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

Specimens were obtained from non-neoplastic colorectal mucosa for RNA ayalysis. Samples were obtained either from surgically resected specimens or during surveillance coloscopy. The expression profiles were determined using Affymetrix Human Genome U133 Plus 2.0 arrays.The correlation between mtDNA CNV and mitochondria-related gene expressions were investigated.

Overall design

Thirty patients who underwent surgical resection or surveillance coloscopy were included in this study. The copy number of mitochondria DNA was measured by absolute quantification using QuantStudio SD digital PCR system. Samples were classified into mtDNA-Low and mtDNA-High at the median.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
30 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

2 linked publications 2 DOIs

Primary publication

  1. Increased Copy Number Variation of mtDNA in an Array-based Digital PCR Assay Predicts Ulcerative Colitis-associated Colorectal Cancer. In vivo (Athens, Greece), 2017

Related publications

  1. Identification of useful genes from multiple microarrays for ulcerative colitis diagnosis based on machine learning methods. Scientific reports, 2022
Available

Metadata Quality Components

Analysis Readiness

Not ready
55% DE readiness

Contrast completeness

10/25 pts

Only one side of the contrast is present; downstream DE needs both groups.

Minimum group size

0/20 pts

Minimum group size cannot be evaluated until a complete contrast exists.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

0/10 pts

Critical warnings must be resolved before downstream analysis.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

  • No complete case/control contrast is available.
  • Critical dataset warnings must be resolved.

Next Steps

  • Resolve critical warnings before downstream analysis.

Analysis Preparation Checklist

4/6 ready

Group labels reviewed

Blocked

No complete case/control grouping exists yet.

Review the sample grouping table and assign both case and control groups.

Minimum sample count met

Blocked

At least one group has fewer than 2 samples.

Choose a larger dataset or merge only scientifically valid groups.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Analysis readiness has blockers that must be resolved before DE.

Analysis preparation checklist contains blocked items.

No complete case/control or treatment/control contrast is available.

At least one comparison group has fewer than two samples.

Critical dataset warnings are present.

Assay context may require expert handling before DESeq2-style analysis.

Study design is model-system or unclear and should be reviewed before analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Ulcerative colitis Disease context came from Normalization rules.
  • Likely case group Ulcerative colitis 30 samples
  • Likely control group No control group detected 0 samples

Intent Interpretation

  • Downgrade or exclusion reason Comparison: Unclear study design. Specimen: Tissue. Comparison suitability: 0%. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Interpreted disease metadata: Ulcerative colitis.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 2 publications; 2 DOIs available: PMID 28652445, PMID 35705632.
  • Publication context: Increased Copy Number Variation of mtDNA in an Array-based Digital PCR Assay Predicts Ulcerative Colitis-associated Colorectal Cancer.. In vivo (Athens, Greece), 2017. PMID 28652445. DOI 10.21873/invivo.11119.
  • Publication context: Identification of useful genes from multiple microarrays for ulcerative colitis diagnosis based on machine learning methods.. Scientific reports, 2022. PMID 35705632. DOI 10.1038/s41598-022-14048-6.

Cautions

  • Comparison: Unclear study design. Specimen: Tissue. Comparison suitability: 0%.
  • Downgraded for tissue search because study design or specimen source is unclear.
  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Unclear study design downgraded for patient tissue tumor-vs-normal search intent.
  • Missing or unclear metadata. Unclear study design downgraded for patient tissue tumor-vs-normal search intent.
  • Microarray expression context downgraded below primary bulk RNA-seq for the default tissue ranking.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Overall score capped because microarray cohorts should be compared separately from RNA-seq cohorts.
  • Missing or unclear metadata. Comparison - Unclear study design. Specimen - Tissue. Comparison suitability: 0%.
  • Missing or unclear metadata. Downgraded for tissue search because study design or specimen source is unclear.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

124 fields

Sample Group Preview

30 rows shown
Show Hide
Study totals: Case 30 | Control 0 | Unassigned 0 | Excluded 0 | Ambiguous 0 30 rows shown
Group Role Accession Title Source / characteristics Reason
Ulcerative colitis Case GSM1915850 Expression data of UC_m01 Patient_01 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915851 Expression data of UC_m02 Patient_02 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915852 Expression data of UC_m03 Patient_03 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915853 Expression data of UC_m04 Patient_04 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915854 Expression data of UC_m05 Patient_05 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915855 Expression data of UC_m06 Patient_06 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915856 Expression data of UC_m07 Patient_07 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915857 Expression data of UC_m08 Patient_08 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915858 Expression data of UC_m09 Patient_09 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915859 Expression data of UC_m10 Patient_10 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915860 Expression data of UC_m11 Patient_11 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915861 Expression data of UC_m12 Patient_12 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915862 Expression data of UC_m13 Patient_13 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915863 Expression data of UC_m14 Patient_14 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915864 Expression data of UC_m15 Patient_15 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915865 Expression data of UC_m16 Patient_16 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915866 Expression data of UC_m17 Patient_17 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915867 Expression data of UC_m18 Patient_18 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915868 Expression data of UC_m19 Patient_19 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915869 Expression data of UC_m20 Patient_20 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915870 Expression data of UC_m21 Patient_21 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915871 Expression data of UC_m22 Patient_22 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915872 Expression data of UC_m23 Patient_23 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915873 Expression data of UC_m24 Patient_24 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915874 Expression data of UC_m25 Patient_25 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915875 Expression data of UC_m26 Patient_26 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915876 Expression data of UC_m27 Patient_27 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-Low Case marker detected.
Ulcerative colitis Case GSM1915877 Expression data of UC_m28 Patient_28 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915878 Expression data of UC_m29 Patient_29 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.
Ulcerative colitis Case GSM1915879 Expression data of UC_m30 Patient_30 Disease state: Ulcerative colitis; Tissue: Colorectal mucosa; Mitochondrial dna copy number: MtDNA-High Case marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.