GSE75916

Expression data of epithelial organoid cultures generated from intestinal mucosa of non-IBD controls and patients with ulcerative colitis

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

The transcriptional signature of mucosa of patients with ulcerative colitis (UC) in remission reveals long-lasting changes in the epithelial barrier which persist once the inflammatory response has resolved. In order to investigate if these changes are caused by primary defects in the epithelial cells, we generated in vitro epithelial organoid cultures (EpOCs) from colon samples of non-IBD controls and UC patients. After induction of differentiation, total RNA was extracted from both EpOCs and differentiated EpOCs (d-EpOCs) and used for hybridization on Affymetrix microarray.

Overall design

Colon samples from non-IBD controls and UC patients were collected. Both surgical and biopsy samples for non-IBD controls, and only biopsy samples for UC patients, were considered. Non-IBD controls were those subjects undergoing surgery for colorectal cancer, colonoscopy for mild gastrointestinal symptoms, or a screening for CRC and who presented no lesions during examination. Inclusion criteria for UC patients at the time of colonoscopy were: age between 18 and 80, diagnosis at least 5 years before inclusion, and exclusion of colitis-associated dysplasia or neoplasia. Endoscopic activity of UC at the time of colonoscopy was categorized according to the Mayo endoscopy subscore. Active disease was defined as Mayo endoscopic subscore of ≥1; quiescent disease was defined as a Mayo score of 0. Only samples from UC patients with inactive or mildly active disease were included in the generation of epithelial organoid cultures (EpOCs). Once expanded, EpOCs were induced to differentiate into the different epithelial lineages (d-EpOCs). Finally, a total of 19 human samples were analysed: 11 from non-IBD controls and 8 from UC patients. From these samples 19 EpOCs and 17 d-EpOCs were generated. RNA was then extracted and used for hybridization on Affymetrix microarrays.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
36 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

1 linked publication 1 DOI

Primary publication

  1. Alterations in the epithelial stem cell compartment could contribute to permanent changes in the mucosa of patients with ulcerative colitis. Gut, 2017
Available

Metadata Quality Components

Analysis Readiness

Review before analysis
85% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

3/15 pts

Unassigned or review-needed samples remain before analysis.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

  • 36 samples need manual grouping review.

Next Steps

  • Resolve unassigned or review-needed samples in the grouping table.

Analysis Preparation Checklist

4/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Needs review

36 review-needed samples and 0 unassigned samples remain.

Resolve ambiguous and unassigned samples before finalizing the contrast.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Analysis readiness has blockers that must be resolved before DE.

One or more samples were flagged for manual review.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Colorectal cancer, inflammatory bowel disease, ulcerative colitis Disease context came from Normalization rules.
  • Likely case group Ulcerative colitis 15 samples
  • Likely control group Control 21 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded for tissue search because it is a model-system experiment. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Organoid. Comparison suitability: 0%.
  • Interpreted disease metadata: Colorectal cancer, Inflammatory bowel disease, Ulcerative colitis.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 1 publication; 1 DOI available: PMID 27803115.
  • Publication context: Alterations in the epithelial stem cell compartment could contribute to permanent changes in the mucosa of patients with ulcerative colitis.. Gut, 2017. PMID 27803115. DOI 10.1136/gutjnl-2016-312609.

Cautions

  • Downgraded for tissue search because it is a model-system experiment.
  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Excluded sample type flags reduce suitability.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Organoid or cell-line experiment downgraded for patient tissue search intent.
  • Microarray expression context downgraded below primary bulk RNA-seq for the default tissue ranking.
  • Excluded model-system sample markers reduce suitability.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Overall score capped because microarray cohorts should be compared separately from RNA-seq cohorts.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

201 fields

Sample Group Preview

36 rows shown
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Study totals: Case 15 | Control 21 | Unassigned 0 | Excluded 0 | Ambiguous 0 36 rows shown
Group Role Accession Title Source / characteristics Reason
Ulcerative colitis Case GSM1970196 EpOC from UC patient [80S] EpOC from biopsy sample of UC patient Case_id: 80; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 16; Age (years): 51 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970197 D-EpOC from UC patient [80D] D-EpOC from biopsy sample of UC patient Case_id: 80; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 16; Age (years): 51 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970198 EpOC from UC patient [88S] EpOC from biopsy sample of UC patient Case_id: 88; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 17; Age (years): 38 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970203 EpOC from UC patient [91S] EpOC from biopsy sample of UC patient Case_id: 91; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 11; Age (years): 54 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970204 D-EpOC from UC patient [91D] D-EpOC from biopsy sample of UC patient Case_id: 91; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 11; Age (years): 54 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970219 EpOC from UC patient [129S] EpOC from biopsy sample of UC patient Case_id: 129; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 18; Age (years): 37 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970220 D-EpOC from UC patient [129D] D-EpOC from biopsy sample of UC patient Case_id: 129; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 18; Age (years): 37 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970221 EpOC from UC patient [134S] EpOC from biopsy sample of UC patient Case_id: 134; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 17; Age (years): 40 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970222 D-EpOC from UC patient [134D] D-EpOC from biopsy sample of UC patient Case_id: 134; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 17; Age (years): 40 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970223 EpOC from UC patient [136S] EpOC from biopsy sample of UC patient Case_id: 136; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 1; Uc_evolution_time (years): 17; Age (years): 50 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970224 D-EpOC from UC patient [136D] D-EpOC from biopsy sample of UC patient Case_id: 136; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 1; Uc_evolution_time (years): 17; Age (years): 50 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970227 EpOC from UC patient [153S] EpOC from biopsy sample of UC patient Case_id: 153; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 8; Age (years): 43 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970228 D-EpOC from UC patient [153D] D-EpOC from biopsy sample of UC patient Case_id: 153; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 0; Uc_evolution_time (years): 8; Age (years): 43 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970229 EpOC from UC patient [154S] EpOC from biopsy sample of UC patient Case_id: 154; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 1; Uc_evolution_time (years): 8; Age (years): 41 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Ulcerative colitis Case GSM1970230 D-EpOC from UC patient [154D] D-EpOC from biopsy sample of UC patient Case_id: 154; Case_phenotype: Ulcerative colitis (UC) patient; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Endoscopic_mayo_score: 1; Uc_evolution_time (years): 8; Age (years): 41 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970199 EpOC from non-IBD control [89S] EpOC from biopsy sample of non-IBD control Case_id: 89; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Sigmoid colon; Age (years): 30 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970200 D-EpOC from non-IBD control [89D] D-EpOC from biopsy sample of non-IBD control Case_id: 89; Case_phenotype: Non-IBD control; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Age (years): 30 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970201 EpOCs from non-IBD control [90S] EpOC from biopsy sample of non-IBD control Case_id: 90; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Descending colon; Age (years): 53 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970202 D-EpOC from non-IBD control [90D] D-EpOC from biopsy sample of non-IBD control Case_id: 90; Case_phenotype: Non-IBD control; Organoid_culture: D-EpOC; Tissue: Descending colon; Age (years): 53 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970205 EpOC from non-IBD control [92S] EpOC from biopsy sample of non-IBD control Case_id: 92; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Sigmoid colon; Age (years): 31 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970206 D-EpOC from non-IBD control [92D] D-EpOC from biopsy sample of non-IBD control Case_id: 92; Case_phenotype: Non-IBD control; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Age (years): 31 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970207 EpOC from non-IBD control [94S] EpOC from surgical sample of non-IBD control Case_id: 94; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Sigmoid colon; Age (years): 48 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970208 D-EpOC from non-IBD control [94D] D-EpOC from surgical sample of non-IBD control Case_id: 94; Case_phenotype: Non-IBD control; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Age (years): 48 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970209 EpOC from non-IBD control [112S] EpOC from biopsy sample of non-IBD control Case_id: 112; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Sigmoid colon; Age (years): 66 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970210 D-EpOC from non-IBD control [112D] D-EpOC from biopsy sample of non-IBD control Case_id: 112; Case_phenotype: Non-IBD control; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Age (years): 66 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970211 EpOC from non-IBD control [113S] EpOC from biopsy sample of non-IBD control Case_id: 113; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Sigmoid colon; Age (years): 57 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970212 D-EpOC from non-IBD control [113D] D-EpOC from biopsy sample of non-IBD control Case_id: 113; Case_phenotype: Non-IBD control; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Age (years): 57 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970213 EpOC from non-IBD control [115S] EpOC from surgical sample of non-IBD control Case_id: 115; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Sigmoid colon; Age (years): 64 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970214 D-EpOC from non-IBD control [115D] D-EpOC from surgical sample of non-IBD control Case_id: 115; Case_phenotype: Non-IBD control; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Age (years): 64 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970215 EpOC from non-IBD control [117S] EpOC from surgical sample of non-IBD control Case_id: 117; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Sigmoid colon; Age (years): 52 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970216 D-EpOC from non-IBD control [117D] D-EpOC from surgical sample of non-IBD control Case_id: 117; Case_phenotype: Non-IBD control; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Age (years): 52 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970217 EpOC from non-IBD control [128S] EpOC from surgical sample of non-IBD control Case_id: 128; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Sigmoid colon; Age (years): 77 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970218 D-EpOC from non-IBD control [128D] D-EpOC from surgical sample of non-IBD control Case_id: 128; Case_phenotype: Non-IBD control; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Age (years): 77 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970225 EpOC from non-IBD control [149S] EpOC from surgical sample of non-IBD control Case_id: 149; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Sigmoid colon; Age (years): 56 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970226 D-EpOC from non-IBD control [149D] D-EpOC from surgical sample of non-IBD control Case_id: 149; Case_phenotype: Non-IBD control; Organoid_culture: D-EpOC; Tissue: Sigmoid colon; Age (years): 56 organoid: Standalone model-system marker retained for comparison grouping: organoid.
Control Control GSM1970231 EpOC from non-IBD control [155S] EpOC from surgical sample of non-IBD control Case_id: 155; Case_phenotype: Non-IBD control; Organoid_culture: EpOC; Tissue: Sigmoid colon; Age (years): 57 organoid: Standalone model-system marker retained for comparison grouping: organoid.

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