GSE81266

Ileal pouch transcriptomics reveal shared pathogenesis between pouchitis and ulcerative colitis

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

71 case / 2 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

UC pouchitis is a potential model of UC. We prospectively examined the pouch transcriptomes of UC and familial adenomatous polyposis (FAP) IPAA patients to unveil molecular mechanisms of UC pouchitis susceptibility. Methods: Total RNA was isolated using the AllPrep DNA/RNA Mini Kit (QIAGEN, Cat No. 8020). RNA quality was evaluated using Bioanalyzer (Agilent, Santa Clara, CA). All RNA samples displayed RNA Integrity Number (RIN) >7. RNAseq including cDNA library preparation was processed at the Genomics Core Facility of University of Chicago (https://fgf.uchicago.edu/). Total RNA in the amount of 100-500μg per sample was depleted of ribosomal RNA using the Ribo-Zero kit (Epicentre, Madison, WI). The directional (first strand) cDNA libraries were prepared following the guide of TruSeq Stranded Total RNA Sample Preparation kit. Results: Unlike FAP patients, UC subjects exhibited a large set of differentially expressed genes (DEGs) between pouch and pre-pouch mucosa as early as 4 months after pouch functionalization. Functional pathway analysis of DEGs in UC pouch revealed: (1) Gain of colon-associated gene expressions and loss of ileum associated gene expressions, (2) enhanced state of immune/inflammatory response, and (3) suppressed xenobiotic, lipid, and bile acid metabolic pathways. These changes were corroborated upon reanalysis of a published larger cross-sectional study of UC and FAP patients. Moreover, >70% of DEGs mapped to published IBD and normal colonic microarray datasets displayed directional changes consistent with active UC, but not Crohn's disease. Conclusions: UC patients exhibit a unique transcriptomic response to ileal pouch creation that can be observed well before disease. The transcriptome alterations provide insights into pouchitis

Overall design

Seventeen patients with UC and four patients with FAP were recruited at the University of Chicago and the Mayo Clinic Rochester. All patients underwent a total proctocolectomy with ileal pouch anal anastomosis (IPAA) as a standard of care. UC patients underwent a pouchoscopy for biopsy of the pre-pouch ileum and pouch at 4 months, 8 months, and 12 months after ileostomy closure. None of these patients had pouchitis.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
77 GSM samples
Primary source
recount3

Trusted source evidence for processed count availability.

recount3 project ID
srp074739
Available
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

1 linked publication 1 DOI

Primary publication

  1. Early Transcriptomic Changes in the Ileal Pouch Provide Insight into the Molecular Pathogenesis of Pouchitis and Ulcerative Colitis. Inflammatory bowel diseases, 2017
Available

Metadata Quality Components

Analysis Readiness

Review before analysis
85% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

3/15 pts

Unassigned or review-needed samples remain before analysis.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

  • 4 samples are still unassigned.

Next Steps

  • Resolve unassigned or review-needed samples in the grouping table.

Analysis Preparation Checklist

4/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Needs review

0 review-needed samples and 4 unassigned samples remain.

Resolve ambiguous and unassigned samples before finalizing the contrast.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Analysis readiness has blockers that must be resolved before DE.

One or more samples are unassigned and need manual grouping decisions.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Crohn's disease, inflammatory bowel disease, ulcerative colitis Disease context came from Normalization rules.
  • Likely case group Ulcerative colitis 71 samples
  • Likely control group Healthy control 2 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded assay: microarray cohorts are separated from the RNA-seq intent. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Biopsy. Comparison suitability: 0%.
  • Interpreted disease metadata: Crohn's disease, Inflammatory bowel disease, Ulcerative colitis.
  • Processed data is available for downstream review.
  • External evidence: recount3 reports trusted processed count availability.
  • External evidence: PubMed links 1 publication; 1 DOI available: PMID 28221248.
  • Publication context: Early Transcriptomic Changes in the Ileal Pouch Provide Insight into the Molecular Pathogenesis of Pouchitis and Ulcerative Colitis.. Inflammatory bowel diseases, 2017. PMID 28221248. DOI 10.1097/MIB.0000000000001027.

Cautions

  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Microarray expression context downgraded below primary bulk RNA-seq for the default tissue ranking.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Overall score capped because microarray cohorts should be compared separately from RNA-seq cohorts.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

230 fields

Sample Group Preview

showing 56 of 77 preview rows
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Study totals: Case 71 | Control 2 | Unassigned 4 | Excluded 0 | Ambiguous 0 showing 56 of 77 preview rows
Group Role Accession Title Source / characteristics Reason
Ulcerative colitis Case GSM2147872 EC1 P500.5 Patient: P500; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 4mo; Age: 28; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147873 EC2 P500.5 Patient: P500; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Prepouch ileum; Biopsytime: 4mo; Age: 28; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147874 EC3 P500.8 Patient: P500; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 8mo; Age: 28; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147875 EC4 P500.8 Patient: P500; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Prepouch ileum; Biopsytime: 8mo; Age: 28; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147876 EC5 P500.12 Patient: P500; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 12mo; Age: 28; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147877 EC6 P500.12 Patient: P500; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Prepouch ileum; Biopsytime: 12mo; Age: 28; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147878 EC7 P502.4 Patient: P502; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 4mo; Age: 39; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147879 EC8 P502.4 Patient: P502; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Prepouch ileum; Biopsytime: 4mo; Age: 39; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147880 EC9 P502.8 Patient: P502; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 8mo; Age: 39; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147881 EC10 P502.8 Patient: P502; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Prepouch ileum; Biopsytime: 8mo; Age: 39; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147882 EC11 P502.12 Patient: P502; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 12mo; Age: 39; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147883 EC12 P502.12 Patient: P502; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Prepouch ileum; Biopsytime: 12mo; Age: 39; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147884 EC13 P602 Patient: P602; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: >12mo; Age: Missing; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147885 EC14 P602 Patient: P602; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: >12mo; Age: Missing; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147886 EC15 P603 Patient: P603; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: >12mo; Age: Missing; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147887 EC16 P603 Patient: P603; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: >12mo; Age: Missing; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147888 NH10 P501.5 Patient: P501; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 4mo; Age: 25; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147889 NH11 P501.6 Patient: P501; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 8mo; Age: 25; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147890 NH12 P501.7 Patient: P501; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 12mo; Age: 25; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147891 NH13 P503.5 Patient: P503; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 4mo; Age: 30; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147892 NH14 P503.6 Patient: P503; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 8mo; Age: 30; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147893 NH15 P503.7 Patient: P503; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 12mo; Age: 30; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147894 NH17 P503.6 Patient: P503; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 8mo; Age: 30; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147895 NH18 P503.7 Patient: P503; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 12mo; Age: 30; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147896 NH19 P505.6 Patient: P505; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 8mo; Age: 47; Sex: Male; Ethnicity: South Indian Case marker detected.
Ulcerative colitis Case GSM2147897 NH1 P427.5 Patient: P427; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 4mo; Age: 30; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147898 NH20 P505.7 Patient: P505; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 12mo; Age: 47; Sex: Male; Ethnicity: South Indian Case marker detected.
Ulcerative colitis Case GSM2147899 NH21 P505.5 Patient: P505; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 4mo; Age: 47; Sex: Male; Ethnicity: South Indian Case marker detected.
Ulcerative colitis Case GSM2147900 NH22 P505.6 Patient: P505; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 8mo; Age: 47; Sex: Male; Ethnicity: South Indian Case marker detected.
Ulcerative colitis Case GSM2147901 NH23 P505.7 Patient: P505; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 12mo; Age: 47; Sex: Male; Ethnicity: South Indian Case marker detected.
Ulcerative colitis Case GSM2147902 NH24 P506.5 Patient: P506; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Prepouch ileum; Biopsytime: 4mo; Age: 30; Sex: Female; Ethnicity: Africa American Case marker detected.
Ulcerative colitis Case GSM2147903 NH26 P506.7 Patient: P506; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Prepouch ileum; Biopsytime: 12mo; Age: 30; Sex: Female; Ethnicity: Africa American Case marker detected.
Ulcerative colitis Case GSM2147904 NH27 P506.5 Patient: P506; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 4mo; Age: 30; Sex: Female; Ethnicity: Africa American Case marker detected.
Ulcerative colitis Case GSM2147905 NH28 P506.6 Patient: P506; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 8mo; Age: 30; Sex: Female; Ethnicity: Africa American Case marker detected.
Ulcerative colitis Case GSM2147906 NH29 P506.7 Patient: P506; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 12mo; Age: 30; Sex: Female; Ethnicity: Africa American Case marker detected.
Ulcerative colitis Case GSM2147907 NH2 P427.6 Patient: P427; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 8mo; Age: 30; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147908 NH30 P507.5 Patient: P507; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Prepouch ileum; Biopsytime: 4mo; Age: 21; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147909 NH32 P507.5 Patient: P507; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 4mo; Age: 21; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147910 NH34 P508.5 Patient: P508; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 4mo; Age: 26; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147911 NH35 P508.6 Patient: P508; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 8mo; Age: 26; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147912 NH36 P508.5 Patient: P508; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 4mo; Age: 26; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147913 NH37 P508.6 Patient: P508; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 8mo; Age: 26; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147914 NH38 P509.5 Patient: P509; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 4mo; Age: 18; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147915 NH39 P509.5 Patient: P509; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 4mo; Age: 18; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147916 NH3 P427.7 Patient: P427; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 12mo; Age: 30; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147917 NH40 P510.5 Patient: P510; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Prepouch ileum; Biopsytime: 4mo; Age: 51; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147918 NH41 P510.5 Patient: P510; Diagnosis: Ulcerative colitis; Prognosis: Pouchitis; Tissue: Ileal pouch; Biopsytime: 4mo; Age: 51; Sex: Female; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147919 NH42 P511.5 Patient: P511; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 4mo; Age: 38; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147920 NH43 P511.6 Patient: P511; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Prepouch ileum; Biopsytime: 8mo; Age: 38; Sex: Male; Ethnicity: Caucasian Case marker detected.
Ulcerative colitis Case GSM2147921 NH44 P511.5 Patient: P511; Diagnosis: Ulcerative colitis; Prognosis: Healthy; Tissue: Ileal pouch; Biopsytime: 4mo; Age: 38; Sex: Male; Ethnicity: Caucasian Case marker detected.
21 additional case samples are hidden from this preview.
Healthy control Control GSM2147947 NH64 Ileum1 Patient: IL1; Diagnosis: Normal; Prognosis: Healthy; Tissue: Ile; Biopsytime: NA; Age: Missing; Sex: Missing; Ethnicity: Caucasian Sample-local control marker detected.
Healthy control Control GSM2147948 NH65 Ileum2 Patient: IL2; Diagnosis: Normal; Prognosis: Healthy; Tissue: Ile; Biopsytime: NA; Age: Missing; Sex: Missing; Ethnicity: Caucasian Sample-local control marker detected.
Unassigned Unassigned GSM2147940 NH62 P701 Patient: P701; Diagnosis: FAP; Prognosis: FAP; Tissue: Prepouch ileum; Biopsytime: >12mo; Age: Missing; Sex: Missing; Ethnicity: Caucasian No explicit case or control marker detected.
Unassigned Unassigned GSM2147941 NH63 P701 Patient: P701; Diagnosis: FAP; Prognosis: FAP; Tissue: Ileal pouch; Biopsytime: >12mo; Age: Missing; Sex: Missing; Ethnicity: Caucasian No explicit case or control marker detected.
Unassigned Unassigned GSM2147942 NH54 P601 Patient: P601; Diagnosis: FAP; Prognosis: FAP; Tissue: Prepouch ileum; Biopsytime: >12mo; Age: Missing; Sex: Male; Ethnicity: Caucasian No explicit case or control marker detected.
Unassigned Unassigned GSM2147943 NH55 P601 Patient: P601; Diagnosis: FAP; Prognosis: FAP; Tissue: Ileal pouch; Biopsytime: >12mo; Age: Missing; Sex: Male; Ethnicity: Caucasian No explicit case or control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.