GSE9452

Definition of an ulcerative colitis preinflammatory state

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

8 case / 18 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

The samples are a part of a study aiming at diagnosing ulcerative colitis from genome-wide gene expression analysis of the colonic mucosa. Colonic mucosal samples were collected as endoscopic pinch biopsies from ulcerative colitis patients and from control subjects. Samples with and without macroscopic signs of inflammation were collected from the patients. Keywords: Disease state analysis

Overall design

The series contain eight UC samples with macroscopic signs of inflammation, 13 UC smaples without macroscopic signs of inflammation, five control subjects.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
26 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

5 linked publications 5 DOIs

Primary publication

  1. Diagnosis of ulcerative colitis before onset of inflammation by multivariate modeling of genome-wide gene expression data. Inflammatory bowel diseases, 2009

Related publications

  1. Identification and analysis of key genes associated with ulcerative colitis based on DNA microarray data. Medicine, 2018
  2. Artemisinin Alleviates Intestinal Inflammation and Metabolic Disturbance in Ulcerative Colitis Rats Induced by DSS. Evidence-based complementary and alternative medicine : eCAM, 2022
  3. Bioinformatics Analysis of Key Candidate Genes and Pathways in Ulcerative Colitis. Biological & pharmaceutical bulletin, 2020
  4. Evaluation of the Mechanism of Sinomenii Caulis in Treating Ulcerative Colitis based on Network Pharmacology and Molecular Docking. Current computer-aided drug design, 2024
Available

Metadata Quality Components

Analysis Readiness

Ready after review
97% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Ulcerative colitis Disease context came from Normalization rules.
  • Likely case group Inflamed tissue 8 samples
  • Likely control group Non-inflamed tissue 18 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded assay: microarray cohorts are separated from the RNA-seq intent. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Biopsy. Comparison suitability: 0%.
  • Interpreted disease metadata: Ulcerative colitis.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 5 publications; 5 DOIs available: PMID 19177426, PMID 29794741, PMID 33132321, PMID 35497913, PMID 37078344.
  • Publication context: Diagnosis of ulcerative colitis before onset of inflammation by multivariate modeling of genome-wide gene expression data.. Inflammatory bowel diseases, 2009. PMID 19177426. DOI 10.1002/ibd.20879.
  • Publication context: Identification and analysis of key genes associated with ulcerative colitis based on DNA microarray data.. Medicine, 2018. PMID 29794741. DOI 10.1097/MD.0000000000010658.
  • Publication context: Bioinformatics Analysis of Key Candidate Genes and Pathways in Ulcerative Colitis.. Biological & pharmaceutical bulletin, 2020. PMID 33132321. DOI 10.1248/bpb.b20-00488.
  • Publication context: 2 additional linked PubMed publications are available.

Cautions

  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

155 fields

Sample Group Preview

26 rows shown
Show Hide
Study totals: Case 8 | Control 18 | Unassigned 0 | Excluded 0 | Ambiguous 0 26 rows shown
Group Role Accession Title Source / characteristics Reason
Inflamed tissue Case GSM239617 Colonic_mucosa_inflammation_26 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, macroscopic inflammation vissible Case marker detected.
Inflamed tissue Case GSM239618 Colonic_mucosa_inflammation_30 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, macroscopic inflammation vissible Case marker detected.
Inflamed tissue Case GSM239714 Colonic_mucosa_inflamation_76 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, macroscopic inflammation vissible Case marker detected.
Inflamed tissue Case GSM239716 Colonic_mucosa_inflamation_93 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, macroscopic inflammation vissible Case marker detected.
Inflamed tissue Case GSM239717 Colonic_mucosa_inflamation_94 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, macroscopic inflammation vissible Case marker detected.
Inflamed tissue Case GSM239718 Colonic_mucosa_inflamation_95 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, macroscopic inflammation vissible Case marker detected.
Inflamed tissue Case GSM239719 Colonic_mucosa_inflamation_119 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, macroscopic inflammation vissible Case marker detected.
Inflamed tissue Case GSM239720 Colonic_mucosa_inflamation_125 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, macroscopic inflammation vissible Case marker detected.
Non-inflamed tissue Control GSM239723 Colonoc_mucosa_non_inflamed_30 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM239725 Colonic_mucosa_non_inflamed_75 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM239726 Colonic_mucosa_non_inflamed_93 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM239727 Colonic_mucosa_non_inflamed_94 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM239729 Colonic_mucosa_non_inflamed_95 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM239730 Colonic_mucosa_non_inflamed_122 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM239731 Colonic_mucosa_non_inflamed_123 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM239732 Colonic_mucosa_non_inflamed_125 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM240022 Colonic_mucosa_non_inflamed_76 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the descending colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM240023 Colonic_mucosa_non_inflamed_721 Colonic mucosal endoscopic pinch biopsy Control subject, biopsy taken from the colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM240024 Colonic_mucosa_non_inflamed_722 Colonic mucosal endoscopic pinch biopsy Control subject, biopsy taken from the colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM240025 Colonic_mucosa_non_inflamed_728 Colonic mucosal endoscopic pinch biopsy Control subject, biopsy taken from the sigmoideum, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM240026 Colonic_mucosa_non_inflamed_741 Colonic mucosal endoscopic pinch biopsy Control subject, biopsy taken from the colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM240027 Colonic_mucosa_non_inflamed_743 Colonic mucosal endoscopic pinch biopsy Control subject, biopsy taken from the sigmoideum, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM240028 Colonic_mucosa_non_inflamed_744 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the colon, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM240029 Colonic_mucosa_non_inflamed_758 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the sigmoideum, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM240030 Colonic_mucosa_non_inflamed_877 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the sigmoideum, no macroscopic signs of inflammation. Sample-local control marker detected.
Non-inflamed tissue Control GSM240031 Colonic_mucosa_non_inflamed_881 Colonic mucosal endoscopic pinch biopsy Ulcerative colitis patient, biopsy taken from the sigmoideum, no macroscopic signs of inflammation. Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.