GSE95095

Differential Gene Expression between involved and uninvolved sites of Crohn’s disease: Insights into a Distinctive Pathogenesis Profile

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

48 case / 12 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

Our objective was to elucidate patterns of gene expression underlying the progression of CD disease. Surgery biopsies (n=60) were assayed using the Illumina cDNA-mediated Annealing, Selection, Extension, and Ligation (DASL) microarray process specifically designed for use with Formalin-Fixed, Paraffin-Embedded (FFPE) clinical samples. Tissue was sampled in consecutive active involved and uninvolved sites at the same time in individual CD patients, and these samples (CD involved and CD uninvolved) were compared with each other as well as to non-inflammatory bowel disease (non-IBD) healthy controls.

Overall design

Gene expression of CD involved and uninvolved is compared at a global level using total RNA from the same CD pateints using the Illumina microarray platform.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
60 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

10 linked publications 10 DOIs

Related publications

  1. CXCL8 as a Potential Biomarker for Crohn's Disease is Associated with Immune Infiltration and RNA Methylation. Biochemical genetics, 2023
  2. Integrated multiple microarray studies by robust rank aggregation to identify immune-associated biomarkers in Crohn's disease based on three machine learning methods. Scientific reports, 2023
  3. Cross talk between bacterial and human gene networks enriched using ncRNAs in IBD disease. Scientific reports, 2023
  4. Identification of ubiquitination-related key biomarkers and immune infiltration in Crohn's disease by bioinformatics analysis and machine learning. Scientific reports, 2025
  5. Integrated Multiomics Elucidates Molecular Mechanisms of Bisphenol A in Exacerbating Crohn's Disease. Mediators of inflammation, 2026
  6. Uncovering common disease mechanisms and critical biomarkers in Crohn's disease with concurrent psoriasis and exploring potential therapeutic agents. PloS one, 2025
  7. C-X-C Motif Chemokine 12 Was Identified as a Potential Gene Target in the Treatment of Crohn's Disease. International journal of general medicine, 2024
  8. Neutrophil Infiltration Characterized by Upregulation of S100A8, S100A9, S100A12 and CXCR2 Is Associated With the Co-Occurrence of Crohn's Disease and Peripheral Artery Disease. Frontiers in immunology, 2022
  9. Identification and validation of the common pathogenesis and hub biomarkers in Hirschsprung disease complicated with Crohn's disease. Frontiers in immunology, 2022
  10. Analysis of ferroptosis-associated genes in Crohn's disease based on bioinformatics. Frontiers in medicine, 2022
Available

Metadata Quality Components

Analysis Readiness

Ready after review
97% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Crohn's disease, inflammatory bowel disease Disease context came from Normalization rules.
  • Likely case group Crohn's disease 48 samples
  • Likely control group Healthy control 12 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded assay: microarray cohorts are separated from the RNA-seq intent. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Tissue. Comparison suitability: 0%.
  • Interpreted disease metadata: Crohn's disease, Inflammatory bowel disease.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 10 publications; 10 DOIs available: PMID 35795659, PMID 36248794, PMID 36714107, PMID 36792688, PMID 37169818.
  • Publication context: Neutrophil Infiltration Characterized by Upregulation of S100A8, S100A9, S100A12 and CXCR2 Is Associated With the Co-Occurrence of Crohn's Disease and Peripheral Artery Disease.. Frontiers in immunology, 2022. PMID 35795659. DOI 10.3389/fimmu.2022.896645.
  • Publication context: Identification and validation of the common pathogenesis and hub biomarkers in Hirschsprung disease complicated with Crohn's disease.. Frontiers in immunology, 2022. PMID 36248794. DOI 10.3389/fimmu.2022.961217.
  • Publication context: Analysis of ferroptosis-associated genes in Crohn's disease based on bioinformatics.. Frontiers in medicine, 2022. PMID 36714107. DOI 10.3389/fmed.2022.1058076.
  • Publication context: 7 additional linked PubMed publications are available.

Cautions

  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Microarray expression context downgraded below primary bulk RNA-seq for the default tissue ranking.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Overall score capped because microarray cohorts should be compared separately from RNA-seq cohorts.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

124 fields

Sample Group Preview

60 rows shown
Show Hide
Study totals: Case 48 | Control 12 | Unassigned 0 | Excluded 0 | Ambiguous 0 60 rows shown
Group Role Accession Title Source / characteristics Reason
Crohn's disease Case GSM2496058 CD_involved_1 Crohn’s disease involved Disease state: CD; Individual id: 1; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496059 CD_involved_2 Crohn’s disease involved Disease state: CD; Individual id: 2; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496060 CD_involved_3 Crohn’s disease involved Disease state: CD; Individual id: 3; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496061 CD_involved_4 Crohn’s disease involved Disease state: CD; Individual id: 4; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496062 CD_involved_5 Crohn’s disease involved Disease state: CD; Individual id: 5; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496063 CD_involved_6 Crohn’s disease involved Disease state: CD; Individual id: 6; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496064 CD_involved_7 Crohn’s disease involved Disease state: CD; Individual id: 7; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496065 CD_involved_8 Crohn’s disease involved Disease state: CD; Individual id: 8; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496066 CD_involved_9 Crohn’s disease involved Disease state: CD; Individual id: 9; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496067 CD_involved_10 Crohn’s disease involved Disease state: CD; Individual id: 10; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496068 CD_involved_11 Crohn’s disease involved Disease state: CD; Individual id: 11; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496069 CD_involved_12 Crohn’s disease involved Disease state: CD; Individual id: 12; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496070 CD_involved_13 Crohn’s disease involved Disease state: CD; Individual id: 13; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496071 CD_involved_14 Crohn’s disease involved Disease state: CD; Individual id: 14; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496072 CD_involved_15 Crohn’s disease involved Disease state: CD; Individual id: 15; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496073 CD_involved_16 Crohn’s disease involved Disease state: CD; Individual id: 16; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496074 CD_involved_17 Crohn’s disease involved Disease state: CD; Individual id: 17; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496075 CD_involved_18 Crohn’s disease involved Disease state: CD; Individual id: 18; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496076 CD_involved_19 Crohn’s disease involved Disease state: CD; Individual id: 19; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496077 CD_involved_20 Crohn’s disease involved Disease state: CD; Individual id: 20; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496078 CD_involved_21 Crohn’s disease involved Disease state: CD; Individual id: 21; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496079 CD_involved_22 Crohn’s disease involved Disease state: CD; Individual id: 22; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496080 CD_involved_23 Crohn’s disease involved Disease state: CD; Individual id: 23; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496081 CD_involved_24 Crohn’s disease involved Disease state: CD; Individual id: 24; Site: Involved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496082 CD_uninvolved_1 Crohn’s disease uninvolved Disease state: CD; Individual id: 1; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496083 CD_uninvolved_2 Crohn’s disease uninvolved Disease state: CD; Individual id: 2; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496084 CD_uninvolved_3 Crohn’s disease uninvolved Disease state: CD; Individual id: 3; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496085 CD_uninvolved_4 Crohn’s disease uninvolved Disease state: CD; Individual id: 4; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496086 CD_uninvolved_5 Crohn’s disease uninvolved Disease state: CD; Individual id: 5; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496087 CD_uninvolved_6 Crohn’s disease uninvolved Disease state: CD; Individual id: 6; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496088 CD_uninvolved_7 Crohn’s disease uninvolved Disease state: CD; Individual id: 7; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496089 CD_uninvolved_8 Crohn’s disease uninvolved Disease state: CD; Individual id: 8; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496090 CD_uninvolved_9 Crohn’s disease uninvolved Disease state: CD; Individual id: 9; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496091 CD_uninvolved_10 Crohn’s disease uninvolved Disease state: CD; Individual id: 10; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496092 CD_uninvolved_11 Crohn’s disease uninvolved Disease state: CD; Individual id: 11; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496093 CD_uninvolved_12 Crohn’s disease uninvolved Disease state: CD; Individual id: 12; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496094 CD_uninvolved_13 Crohn’s disease uninvolved Disease state: CD; Individual id: 13; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496095 CD_uninvolved_14 Crohn’s disease uninvolved Disease state: CD; Individual id: 14; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496096 CD_uninvolved_15 Crohn’s disease uninvolved Disease state: CD; Individual id: 15; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496097 CD_uninvolved_16 Crohn’s disease uninvolved Disease state: CD; Individual id: 16; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496098 CD_uninvolved_17 Crohn’s disease uninvolved Disease state: CD; Individual id: 17; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496099 CD_uninvolved_18 Crohn’s disease uninvolved Disease state: CD; Individual id: 18; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496100 CD_uninvolved_19 Crohn’s disease uninvolved Disease state: CD; Individual id: 19; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496101 CD_uninvolved_20 Crohn’s disease uninvolved Disease state: CD; Individual id: 20; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496102 CD_uninvolved_21 Crohn’s disease uninvolved Disease state: CD; Individual id: 21; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496103 CD_uninvolved_22 Crohn’s disease uninvolved Disease state: CD; Individual id: 22; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496104 CD_uninvolved_23 Crohn’s disease uninvolved Disease state: CD; Individual id: 23; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Crohn's disease Case GSM2496105 CD_uninvolved_24 Crohn’s disease uninvolved Disease state: CD; Individual id: 24; Site: Uninvolved; Tissue: Intestinal tissue Case marker detected.
Healthy control Control GSM2496106 Normal_control_1 Control Disease state: Normal; Individual id: 1; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496107 Normal_control_2 Control Disease state: Normal; Individual id: 2; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496108 Normal_control_3 Control Disease state: Normal; Individual id: 3; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496109 Normal_control_4 Control Disease state: Normal; Individual id: 4; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496110 Normal_control_5 Control Disease state: Normal; Individual id: 5; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496111 Normal_control_6 Control Disease state: Normal; Individual id: 6; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496112 Normal_control_7 Control Disease state: Normal; Individual id: 7; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496113 Normal_control_8 Control Disease state: Normal; Individual id: 8; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496114 Normal_control_9 Control Disease state: Normal; Individual id: 9; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496115 Normal_control_10 Control Disease state: Normal; Individual id: 10; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496116 Normal_control_11 Control Disease state: Normal; Individual id: 11; Tissue: Intestinal tissue Sample-local control marker detected.
Healthy control Control GSM2496117 Normal_control_12 Control Disease state: Normal; Individual id: 12; Tissue: Intestinal tissue Sample-local control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.