GSE96665

Integrated analysis Of Biopsies From Inflammatory Bowel Disease Patients Identifies SAA1 As A Link Between Mucosal Microbes With TH17 And TH22 Cells [diseased subjects]

Whole-study status Not suitable

Dataset does not meet the current suitability threshold.

Whole-study downloads — these do not apply your sample requirements.

Selected cohort requirements

Samples meeting selected requirements: 0 cases / 0 controls

View matching samples

23 case / 18 control samples elsewhere in the study have incomplete or conflicting evidence; they are not included above

Patient pairs verified in indexed sample metadata: 0

A case/control comparison meeting these requirements is not confirmed.

Counts describe samples, not independent patients. Pair verification uses explicit subject identifiers in indexed sample metadata. Zero verified pairs does not establish that the study is unpaired; reported matched-normal design alone is insufficient.

Summary

Inflammatory bowel diseases (IBD) are believed to be driven by dysregulated interactions between the host and the gut microbiota. Our goal is to characterize the relationships between mucosal T cells, the host tissue environment and microbial communities in IBD patients to identify new therapeutic targets. We identified 26 predictors from our combined data set that were effective in distinguishing between regions of the intestine undergoing active inflammation and regions that were normal. Network analysis on these 26 predictors revealed SAA1 as the most connected node linking the abundance of the genus Bacteroides with the production of IL17 and IL22 by CD4+ T cells. The SAA1-linked microbial and transcriptome interactions were further validated with data from the pediatric IBD RISK cohort. This study identifies expression of SAA1 as an important link between mucosal T cells, microbial communities and their tissue environment in IBD patients. A combination of FACS, gene expression and microbial profiling can distinguish between intestinal inflammatory states in IBD regardless of disease types.

Overall design

We characterized mucosal CD4+ T cells using flow cytometry (FACS), along with matching mucosal global gene expression and microbial communities data from 35 pinch biopsy samples from IBD patients. We analyzed these data sets using an integrated framework to identify predictors of inflammatory states and then validated the putative relationship networks by analyzing data from the pediatric RISK cohort.

Source Evidence

4 sources
Source Contribution Status
GEO

Original dataset metadata, sample metadata, source links, and GEO data availability flags.

Sample records
41 GSM samples
Primary source
recount3

Planned trusted source for processed count availability.

No match found
Expression Atlas

Planned source for curated expression experiment links.

No match found
PubMed

Primary GEO citations and related PubMed articles that mention this dataset.

2 linked publications 2 DOIs

Primary publication

  1. Integrated Analysis of Biopsies from Inflammatory Bowel Disease Patients Identifies SAA1 as a Link Between Mucosal Microbes with TH17 and TH22 Cells. Inflammatory bowel diseases, 2017

Related publications

  1. Identification of useful genes from multiple microarrays for ulcerative colitis diagnosis based on machine learning methods. Scientific reports, 2022
Available

Metadata Quality Components

Analysis Readiness

Ready after review
97% DE readiness

Contrast completeness

25/25 pts

Both case and control groups are present in the proposed grouping.

Minimum group size

20/20 pts

Each contrast group has at least 2 samples.

Processed data availability

20/20 pts

Processed data is marked available in the GEO index.

Sample decision clarity

15/15 pts

All samples are assigned to a case or control group.

Blocking warnings

7/10 pts

Warnings are present, but none are critical blockers.

Metadata normalization

10/10 pts

Metadata quality is high enough to support downstream handoff.

Blockers

No readiness blockers detected.

Next Steps

  • Verify the proposed sample assignments and case-versus-control comparison against the GEO metadata before using them in DESeq2 or another differential-expression tool.
  • Confirm processed count files and sample identifiers match the exported metadata.

Analysis Preparation Checklist

5/6 ready

Group labels reviewed

Needs review

Siftome proposed case and control labels, but no reviewer confirmation is stored yet.

Open the sample grouping CSV and confirm group labels before analysis.

Minimum sample count met

Ready

Both groups meet the minimum of 2 samples.

Confirm whether the cohort is large enough for the intended statistical analysis.

Processed counts available

Ready

Processed data availability is marked true in the GEO index.

Verify that downloaded processed files contain usable count or expression identifiers.

Ambiguous samples handled

Ready

No review-needed or unassigned samples are present in the proposed grouping.

Review the grouping table for biologically meaningful differences that the automated checks may not detect.

Exclusion decisions recorded

Ready

No hard-excluded samples were detected by the current rules.

Record any manual exclusions in the downstream analysis notes.

Source traceability preserved

Ready

The dataset and sample accessions can be traced back to GEO.

Keep GEO accessions with exported metadata.

Bioinformatician Review

Bioinformatician review required

Ask a bioinformatician to review grouping, count availability, exclusions, and design before DE.

Assay context may require expert handling before DESeq2-style analysis.

Why This Is Ranked Here

Tumor vs normal
Not suitable Dataset does not meet the current suitability threshold.

Applied Review Decisions

No active review decisions affect this ranking.

Derived Classifications

  • Assay differs from search intent Microarray Search intent expects bulk RNA-seq. Assay came from Normalization rules.
  • Disease context Crohn's disease, inflammatory bowel disease, ulcerative colitis Disease context came from Normalization rules.
  • Likely case group Inflamed tissue 23 samples
  • Likely control group Normal tissue 18 samples

Intent Interpretation

  • Downgrade or exclusion reason Downgraded assay: microarray cohorts are separated from the RNA-seq intent. Derived from current-intent ranking reasons.

Ranking Facts

Supporting evidence

  • Comparison: Disease/model vs control. Specimen: Biopsy. Comparison suitability: 0%.
  • Interpreted disease metadata: Crohn's disease, Inflammatory bowel disease, Ulcerative colitis.
  • Processed data is available for downstream review.
  • External evidence: PubMed links 2 publications; 2 DOIs available: PMID 28806280, PMID 35705632.
  • Publication context: Integrated Analysis of Biopsies from Inflammatory Bowel Disease Patients Identifies SAA1 as a Link Between Mucosal Microbes with TH17 and TH22 Cells.. Inflammatory bowel diseases, 2017. PMID 28806280. DOI 10.1097/MIB.0000000000001208.
  • Publication context: Identification of useful genes from multiple microarrays for ulcerative colitis diagnosis based on machine learning methods.. Scientific reports, 2022. PMID 35705632. DOI 10.1038/s41598-022-14048-6.

Cautions

  • Downgraded assay: microarray cohorts are separated from the RNA-seq intent.
  • Disease/control design without a tumor/normal comparison is outside tumor/normal triage.
  • Overall score capped because comparison suitability is weak for the selected search intent.
  • Comparison suitability score is below the Phase 1 recommended threshold.

Dataset Warnings

Normalized Fields

276 fields

Sample Group Preview

41 rows shown
Show Hide
Study totals: Case 23 | Control 18 | Unassigned 0 | Excluded 0 | Ambiguous 0 41 rows shown
Group Role Accession Title Source / characteristics Reason
Inflamed tissue Case GSM2537668 10I-CD-Active-Ileum-1 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Active; Intestinal region: Ileum Case marker detected.
Inflamed tissue Case GSM2537670 11I-CD-Active-Sigmoid-2 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Active; Intestinal region: Sigmoid Case marker detected.
Inflamed tissue Case GSM2537674 14I-CD-Active-Ileum-5 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Active; Intestinal region: Ileum Case marker detected.
Inflamed tissue Case GSM2537676 15N-UC-Inactive-Transverse-6 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Inactive; Intestinal region: Transverse Case marker detected.
Inflamed tissue Case GSM2537678 18N-CD-Inactive-Sigmoid-8 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Inactive; Intestinal region: Sigmoid Case marker detected.
Inflamed tissue Case GSM2537680 1I-UC-Inactive-Sigmoid-10 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Inactive; Intestinal region: Sigmoid Case marker detected.
Inflamed tissue Case GSM2537682 20N-UC-Inactive-Sigmoid-11 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Inactive; Intestinal region: Sigmoid Case marker detected.
Inflamed tissue Case GSM2537683 21I-UC-Active-Transverse-12 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Active; Intestinal region: Transverse Case marker detected.
Inflamed tissue Case GSM2537688 24I-UC-Inactive-Ascending-15 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Inactive; Intestinal region: Ascending Case marker detected.
Inflamed tissue Case GSM2537689 24N-UC-Active-Sigmoid-15 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Active; Intestinal region: Sigmoid Case marker detected.
Inflamed tissue Case GSM2537691 26I-UC-Inactive-Rectum-17 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Inactive; Intestinal region: Rectum Case marker detected.
Inflamed tissue Case GSM2537692 26N-UC-Inactive-Transverse-17 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Inactive; Intestinal region: Transverse Case marker detected.
Inflamed tissue Case GSM2537693 27I-UC-Active-Sigmoid-18 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Active; Intestinal region: Sigmoid Case marker detected.
Inflamed tissue Case GSM2537694 27N-UC-Inactive-Transverse-18 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Inactive; Intestinal region: Transverse Case marker detected.
Inflamed tissue Case GSM2537697 3Ia-CD-Active-Sigmoid-20 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Active; Intestinal region: Sigmoid Case marker detected.
Inflamed tissue Case GSM2537698 3Ib-CD-Active-Sigmoid-20 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Active; Intestinal region: Sigmoid Case marker detected.
Inflamed tissue Case GSM2537699 4I-CD-Active-Ileum-21 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Active; Intestinal region: Ileum Case marker detected.
Inflamed tissue Case GSM2537701 5I-CD-Active-Descending-22 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Active; Intestinal region: Descending Case marker detected.
Inflamed tissue Case GSM2537702 5N-CD-Inactive-Transverse-22 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Inactive; Intestinal region: Transverse Case marker detected.
Inflamed tissue Case GSM2537704 7N-UC-Active-Sigmoid-24 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Active; Intestinal region: Sigmoid Case marker detected.
Inflamed tissue Case GSM2537705 8Ia-UC-Active-Sigmoid-25 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Active; Intestinal region: Sigmoid Case marker detected.
Inflamed tissue Case GSM2537706 8Ib-UC-Active-Ascending-25 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Active; Intestinal region: Ascending Case marker detected.
Inflamed tissue Case GSM2537707 9I-UC-Active-Ascending-26 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Active; Intestinal region: Ascending Case marker detected.
Normal tissue Control GSM2537669 10N-CD-Normal-Sigmoid-1 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Normal; Intestinal region: Sigmoid Control marker detected.
Normal tissue Control GSM2537671 12I-UC-Normal-Sigmoid-3 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Normal; Intestinal region: Sigmoid Control marker detected.
Normal tissue Control GSM2537672 12N-UC-Normal-Cecum-3 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Normal; Intestinal region: Cecum Control marker detected.
Normal tissue Control GSM2537673 13N-UC-Normal-Transverse-4 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Normal; Intestinal region: Transverse Control marker detected.
Normal tissue Control GSM2537675 14N-CD-Normal-Ileum-5 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Normal; Intestinal region: Ileum Control marker detected.
Normal tissue Control GSM2537677 17N-CD-Normal-Descending-7 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Normal; Intestinal region: Descending Control marker detected.
Normal tissue Control GSM2537679 19N-UC-Normal-Transverse-9 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Normal; Intestinal region: Transverse Control marker detected.
Normal tissue Control GSM2537681 1N-UC-Normal-Cecum-10 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Normal; Intestinal region: Cecum Control marker detected.
Normal tissue Control GSM2537684 22Na-CD-Normal-Sigmoid-13 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Normal; Intestinal region: Sigmoid Control marker detected.
Normal tissue Control GSM2537685 22Nb-CD-Normal-Ascending-13 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Normal; Intestinal region: Ascending Control marker detected.
Normal tissue Control GSM2537686 23Na-CD-Normal-Ascending-14 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Normal; Intestinal region: Ascending Control marker detected.
Normal tissue Control GSM2537687 23Nb-CD-Normal-Descending-14 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Normal; Intestinal region: Descending Control marker detected.
Normal tissue Control GSM2537690 25N-CD-Normal-Sigmoid-16 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Normal; Intestinal region: Sigmoid Control marker detected.
Normal tissue Control GSM2537695 2I-UC-Normal-Rectum-19 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Normal; Intestinal region: Rectum Control marker detected.
Normal tissue Control GSM2537696 2N-UC-Normal-Cecum-19 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Normal; Intestinal region: Cecum Control marker detected.
Normal tissue Control GSM2537700 4N-CD-Normal-Descending-21 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Normal; Intestinal region: Descending Control marker detected.
Normal tissue Control GSM2537703 6N-CD-Normal-Transverse-23 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): CD; Inflammation: Normal; Intestinal region: Transverse Control marker detected.
Normal tissue Control GSM2537708 9N-UC-Normal-Ascending-26 Intestinal mucosa pinch biopsy Disease (uc = ulcerative colitis; Cd = crohn's disease): UC; Inflammation: Normal; Intestinal region: Ascending Control marker detected.

Use as Discovery Cohort

External Validation Finder

Carry Siftome's disease, organism, specimen, assay, data type, and accession into a validation project. Uncertain fields remain Unknown.